| 九里香内生细菌HBS-1的鉴定及其对芒果采后病害的防效 |
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| 引用本文： | 王兰英,廖凤仙,骆焱平.九里香内生细菌HBS-1的鉴定及其对芒果采后病害的防效[J].江苏农业学报,2012(1):41-45. |
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| 作者姓名： | 王兰英 廖凤仙 骆焱平 |
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| 作者单位： | 海南大学热带作物种质资源保护与开发利用教育部重点实验室;海南大学环境与植物保护学院 |
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| 基金项目： | 海南大学热带作物种质资源保护与开发利用教育部重点实验室项目(2010hckled-08),海南大学青年基金(Qnjj1157);海南省自然科学基金项目(311028) |
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| 摘 要： | 通过组织表面消毒法对九里香内生菌进行分离,以芒果炭疽病菌(Colletotrichum gloeosporioides Penz)和芒果蒂腐病菌(Botryodiplodia theobromae Pat)为靶标菌对获得菌株进行皿内拮抗能力筛选,利用浸果法测定菌株发酵滤液对芒果采后病害的防治效果,结合菌株形态、生理生化特征及16S rDNA序列鉴定菌株。结果从九里香中分离到28株内生菌,其中有8株真菌、9株细菌对2种病原菌均有拮抗活性,以HBS-1抑菌活性最好,对芒果炭疽病菌及芒果蒂腐病菌抑制率分别为42.0%和50.0%;菌株HBS-1发酵滤液能明显降低芒果采后病害的发病率,10倍稀释液的防效优于化学药剂特克多800倍液,50倍稀释液的防效与化学药剂特克多800倍液相当;经鉴定,内生菌HBS-1为芽孢杆菌属枯草芽孢杆菌(Bacillus subtilis)。
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| 关 键 词： | 九里香 内生菌 芒果炭疽病菌 芒果蒂腐病菌 防效 |
Identification of an endophytic bacterial strain HBS-1 isolated from Mangifern indica and its biological control efficacy against diseases of postharvest mango |
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| WANG Lan-ying,LIAO Feng-xian,LUO Yan-ping.Identification of an endophytic bacterial strain HBS-1 isolated from Mangifern indica and its biological control efficacy against diseases of postharvest mango[J].Jiangsu Journal of Agricultural Sciences,2012(1):41-45. |
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| Authors: | WANG Lan-ying LIAO Feng-xian LUO Yan-ping |
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| Institution: | (Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Ministry of Education,College of Environment and Plant Protection,Hainan University,Haikou 570228,China) |
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| Abstract: | Endophytic bacteria were isolated by Mangifern indica by surface disinfection method.Their antagonistic activities against Colletotrichum gloeosporioides Penz and Botryodiplodia theobromae Pat were tested to get the best strain.For the strain,control effect of its fermentation liquid filtrate was determined,and identified by morphology,physiological and biochemical characteristics,and 16S rDNA sequence.The results were as follows.8 fungi and 9 bacteria of 28 endophytes from Mangifern indica showed high antagonistic activities,among which HBS-1 displayed 42.0% and 50.0% control efficacy against Colletotrichum gloeosporioides and Botryodiplodia theobromae,respectively.Its fermentation liquid filtrate could significantly reduce the incidences of mango postharvest diseases.By analysing the morphology physiological and biochemical characteristics,and 16S rDNA sequence,HBS-1 strain was identified as Bacillus subtilis. |
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| Keywords: | Mangifern indica endophytic bacterium Colletotrichum gloeosporioides Botryodiplodia theobromae efficacy |
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