中国沙棘体细胞胚胎间接发生与植株再生

以中国沙棘组培苗茎尖为外植体，在1/4 MS基本培养基上，对愈伤组织、胚性愈伤组织及体细胞胚胎进行诱导，探讨植物生长调节剂配比对中国沙棘愈伤组织、胚性愈伤组织的诱导和体细胞胚发生的影响。结果表明:在KT 1.0 mg/L + NAA 0.3 mg/L的最佳组合中，暗培养20 d后，愈伤组织的诱导率达到90.10%；暗培养30 d后，胚性愈伤组织的诱导率达到77.67%；将胚性愈伤组织转移到新鲜诱导培养基上，光培养20 d，平均体细胞胚发生数达到15.48。为了得到完整的再生小植株，将胚状体转移到不含任何生长激素的体细胞胚发育及植株再生培养基上，分析了KT和白砂糖不同浓度的组合对小植株再生的影响。结果发现:WPM + KT 0.05 mg/L + 白砂糖 20 g/L + 琼脂粉 6.5 g/L + CH 0.5 g/L处理上的植株再生率达到60.30%。该研究建立起了完整的中国沙棘体细胞胚胎间接发生的再生体系。 

Indirect somatic embryogenesis and plant regeneration of Hippophae rhamnoides sub.sinensis.

Using the shoot tips of seabuckthorn as explants, the callus, embryogenic callus and somatic embryos were induced on 1/4 MS basal medium and the effects of different plant growth regulator combinations on callus, embryogenic callus induction and somatic embryogenesis were studied. Results showed that the best combination was KT 1.0 mg/L + NAA 0.3 mg/L, on which the induction frequency of callus and embryogenic callus reached 90.10% and 77.67% after 20 and 30 days culturing in darkness, respectively. When the embryogenic callus were transferred to fresh induction medium and cultured under the light, the average number of somatic embryogenesis was 15.48 after 20 days. In order to get the regeneration plantlet, the embryoids were transferred to the medium for somatic embryo development and plant regeneration. The effects of KT combinations and different concentrations of granulated sugaron plant regeneration were analyzed. The frequency of plant regeneration was 60.30% on the medium of WPM + KT 0.05 mg/L + granulated sugar 20 g/L + agar 6.5 g/L + CH 0.5 g/L. In this study, a complete system for plant regeneration via indirect somatic embryogenesis from shoot tips of seabuckthorn was achieved.