| 姜四倍体离体诱导及其形态学分析 |
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| 引用本文： | 王志敏,牛义,宋明,汤青林.姜四倍体离体诱导及其形态学分析[J].中国蔬菜,2010,1(4):41-46. |
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| 作者姓名： | 王志敏 牛义 宋明 汤青林 |
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| 作者单位： | 西南大学园艺园林学院，重庆市蔬菜学重点实验室，重庆 400716 |
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| 基金项目： | 西南大学博士基金项目(20710904) |
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| 摘 要： | 以丛生芽为材料,在离体培养条件下比较了秋水仙素不同浓度、不同处理时间诱导姜体细胞染色体加倍的效果。结果表明:以0.20%秋水仙素液体培养基处理8d的诱导效果最好,诱变率和成活率分别达到了42.86%和70.00%。根尖染色体压片检查发现,四倍体染色体数为2n=4x=44,二倍体对照为2n=2x=22。四倍体与二倍体相比表现出植株高大,茎秆变粗,叶片长、宽、厚均增大,叶片下表皮气孔密度减小,气孔和保卫细胞增大,保卫细胞内叶绿体数增加等特征,其中气孔密度及保卫细胞内的叶绿体数可作为鉴别四倍体与二倍体的重要性状。
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| 关 键 词： | 姜 离体培养 多倍体 形态学 |
| 收稿时间： | 2009-09-18; |
Tetraploid of Zingiber officinale Roscoe.in Vitro Inducement and Its Morphology Analysis |
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| WANG Zhi-min,NIU Yi,SONG Ming,TANG Qing-lin.Tetraploid of Zingiber officinale Roscoe.in Vitro Inducement and Its Morphology Analysis[J].China Vegetables,2010,1(4):41-46. |
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| Authors: | WANG Zhi-min NIU Yi SONG Ming TANG Qing-lin |
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| Institution: | College of Horticulture and Landscape Architecture,Southwest University,Key Lab in Olericulture of Chongqing,Chongqing 400716,China |
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| Abstract: | Taking clustering ginger(Zingiber officinale Roscoe.)shoot as materials,we compared the doubling effects of its somatic cell chromosome under in vitro cultivation condition and with different colchicines concentration and different treating time period.The results showed that the inducement effect was the best,when shoots were treated in liquid culture medium of 0.2 % colchicines concentration for 8 days.The inducement and survival rate were 42.86 % and 70.00 %,respectively.The chromosome number of tetraplo... |
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| Keywords: | Zingiber officinale Roscoe In vitro Polyploid Morphology |
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