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| 蛹虫草鲁山株C0511菌丝液体培养基筛选试验 | |
| 引用本文： | 宋越冬. 蛹虫草鲁山株C0511菌丝液体培养基筛选试验[J]. 河南农业科学, 2010, 0(12) |
| 作者姓名： | 宋越冬 |
| 作者单位： | 山东理工大学,农业工程与食品科学学院,山东,淄博,255049 |
| 基金项目： | 山东理工大学自然科学基金 |
| 摘 要： | 液体培养蛹虫草鲁山菌株,以菌丝体产量为指标,对培养基的碳源、氮源、无机盐、维生素B1进行单因素试验,并在单因素基础上进行四因素三水平正交试验,结果表明:影响液体培养菌丝产量的主次因素依次为氮源、碳源、维生素B1和无机盐。最佳培养基为:蔗糖20 g/L、蛋白胨25 g/L、维生素B10.15 g/L,无机盐为KH2PO41.5 g/L+MgSO40.5 g/L+CaCl20.25 g/L。 |
| 关 键 词： | 蛹虫草 液体培养基 正交试验 |
Selection of Submerged Culture Medium for Cordyceps militaris Strain Lushan C0511 |
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| SONG Yue-dong. Selection of Submerged Culture Medium for Cordyceps militaris Strain Lushan C0511[J]. Journal of Henan Agricultural Sciences, 2010, 0(12) | |
| Authors: | SONG Yue-dong |
| Abstract: | The effects of culture medium composition such as carbon source,nitrogen,mineral salts and vitamine B1 to biomass of Cordyceps militairis were investigated by single factor experiment and 4 factors and 3 levels orthogonal design respectively.The research showed that the order of the factors from primary to secondary was: nitrogen,carbon source,vitamine and mineral salts.And the best medium was 20g/L sucrose+25g/L peptone+0.15g/L vitamine B1+1.5g/L KH2PO4+0.5g/L MgSO4 and 0.25g/L CaCl2. |
| Keywords: | Cordyceps militairis Submerged culture medium Orthogonal design |
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