| 应用套式RT—PCR检测猪瘟制品中牛病毒性腹泻病毒污染的研究 |
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| 引用本文： | 祖立闯,魏凤,苗立中,沈志强. 应用套式RT—PCR检测猪瘟制品中牛病毒性腹泻病毒污染的研究[J]. 养猪, 2011, 0(6): 97-100 |
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| 作者姓名： | 祖立闯 魏凤 苗立中 沈志强 |
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| 作者单位： | 1. 山东绿都生物科技有限公司,山东滨州,256600
2. 山东省滨州畜牧兽医研究院,山东滨州,256600 |
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| 基金项目： | 基金项目:山东省自主创新成果转化重大专项计划 |
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| 摘 要： | 为建立猪瘟制品中牛病毒性腹泻病毒（BVDV）污染的诊断方法，本研究根据GenBank公布的BVDV全基因组序列，选择BVDV保守性比较高的5′非编码区，利用Olig06．0软件设计了2对特异性引物，建立了检测BVDV的套式RT-PCR方法。该方法极大提高了常规RT-PCR检测方法的特异性和敏感性，重复性好、特异性强、敏感性高，可以准确快速检测出极低含量的BVDV，为动物与疫苗生物制品原辅料中BVDV的快速低含量检测提供了一种简单、特异、敏感、快速的检测方法。
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| 关 键 词： | 猪瘟 牛病毒性腹泻病毒 RT—PCR 检测 |
Research of a nest RT-PCR method for detection of bovine viral diarrhea virus in the classical swine fever preparation |
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| ZU Lichuang,WEI Feng,MIAO Lizhong,SHEN Zhiqiang. Research of a nest RT-PCR method for detection of bovine viral diarrhea virus in the classical swine fever preparation[J]. Swine Production, 2011, 0(6): 97-100 |
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| Authors: | ZU Lichuang WEI Feng MIAO Lizhong SHEN Zhiqiang |
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| Affiliation: | ZU Lichuang1,WEI Feng2,MIAO Lizhong2,SHEN Zhiqiang1,2 (1.Shandong Lvdu Bio-Sciences and Technology Co.,Ltd.,Binzhou 256600,China,2.Shandong Binzhou Animal Science and Veterinary Medicine Academy,China) |
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| Abstract: | Based on GenBank published BVDV genome sequence,choosing the BVDV conservative quite high 5′ non-code area,two pairs of primers was designed using Oligo6.0 biology software,a nest RT-PCR detection method was established.This method has good reproducibility,specificity and sensitivity,may diagnosis low content BVDV accurately and rapidly,which enhanced RT-PCR detection method specificity and sensitivity enormously. Clinical initial application showed the nest RT-PCR methods sensitive,especially,quickly,it can be used for animals,raw materials of biological products and vaccines fast low-level detection of BVDV. |
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| Keywords: | classical swine fever virus bovine viral diarrhea virus RT-PCR detection |
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