成熟石榴叶片DNA提取方法研究

目的]为了从成熟石榴叶片中提取高质量、高产量的基因组DNA。方法]针对成熟石榴叶片含多糖、多酚的特性，用改良CTABI及改良CTABⅡ法分别提取2个品种（墨石榴和青皮软籽石榴）的成熟石榴鲜叶、冻叶、干叶基因组DNA，并通过琼脂糖凝胶电泳、紫外分光光度计、酶切鉴定。【结果]改良CrABⅡ法提取的基因组DNA电泳时点样孔干净，无拖带，OD260nm／OD280nm为1．7—1．9，酶切完全，其质量、产量都高于改良CTABI法。结论]改良CTABⅡ法是提取成熟石榴叶片中高质量、高产量基因组DNA的有效方法。

Study on DNA Extraction Methods of Mature Pomegranate (Punica granatum Linn.) Leaves

Objective] The research aimed to extract the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves. Method ] According to the character of containing amylase and polyphenol, genomic ONA in fresh leaves, frozen leaves and dry leaves of 2 kinds of mature pomegranates were extracted by improved CTAB I and CTAB U method. And they were identified by agarose gel elec-trophoresis,ultraviolet spectrophotometer method and enzyme digestion. Result] The results showed that the sample hole was clean and no pulling, OD_(260nm)/OD_(280nm) was 1.7-1.9 when the genomic DNA extracted by improved CTAB II method was electrophoresis. And its quality and yield was high than that extracted by improved CTAB I method. Conclusion] The improved CTAB H method was the effective method of extracting the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves.