| 木聚糖酶高产菌株的诱变选育及产酶条件的研究 |
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| 引用本文： | 侯伯男,马佳宁,单安山.木聚糖酶高产菌株的诱变选育及产酶条件的研究[J].东北农业大学学报,2009,40(7). |
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| 作者姓名： | 侯伯男 马佳宁 单安山 |
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| 作者单位： | 东北农业大学动物营养研究所,哈尔滨,150030 |
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| 基金项目： | “十一五”国家科技支撑计划子课题(2006BAD12B0-7-11);;黑龙江省科技攻关重大资助项目(GA07B201);;东北农业大学创新团队基金项目 |
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| 摘 要： | 试验对黑曲霉进行紫外诱变,结合透明圈法和DNS法从中筛选出一株酶活较高的突变株N86,并对其液体发酵培养基组分进行研究。结果表明,突变株N86的液体发酵培养基最优成分为:麸皮2g,硫酸铵1.0%,葡萄糖0.1%,磷酸二氢钾0.2%,七水合硫酸镁0.05%,吐温-80为0.1%,培养基pH6,250mL三角瓶装液50mL,突变株酶活最高达139IU·mL-1,产酶高峰在72h左右。
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| 关 键 词： | 黑曲霉 木聚糖酶 高产菌株 产酶条件 |
Mutation of the strain highly producing xylanase and its enzyme production conditions studly |
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| HOU Bonan,MA Jianing,SHAN Anshan.Mutation of the strain highly producing xylanase and its enzyme production conditions studly[J].Journal of Northeast Agricultural University,2009,40(7). |
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| Authors: | HOU Bonan MA Jianing SHAN Anshan |
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| Institution: | Institute of Animal Nutrition;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | The research was conducted to study optimization liquid culture medium of Aspergillus niger N86. Aspergillus niger N86, a strain of high xylanase yielding bacteria was screened by the way clear halo comparison and DNS from the products of ultraviolet mutating Aspergillus niger. The result indicated: the optimum component of liquid culture medium was: wheat bran 2 g, ammonium sulfate 1.0%, glucose 0.1%, KH2PO4 0.2%, MgSO4.7H2O 0.05%, Tween-80 0.1%, the initial of liquid culture medium pH 6, 50 mL/250 mL flask, and the activity of mutant was up to 139 IU·mL-1 at most. |
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| Keywords: | Aspergillus niger xylanase highlyprocucing xylanase strain enzyme producing condition |
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