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截形叶螨抗哒螨灵种群抗性遗传方式的研究
引用本文:宋丽雯,张君霞,杨顺义,沈慧敏.截形叶螨抗哒螨灵种群抗性遗传方式的研究[J].植物保护,2016,42(1):112-115.
作者姓名:宋丽雯  张君霞  杨顺义  沈慧敏
作者单位:1. 北京市农林科学院植物保护环境保护研究所,北京,100097;2. 北京农学院植物科学技术学院,北京,102206
基金项目:公益性行业(农业)科研专项(201003065); 北京市农林科学院青年科研基金(QNJJ 201320)
摘    要:通过对室内选育出的截形叶螨敏感品系和抗哒螨灵品系的杂交和回交试验表明:所测得的截形叶螨正交F1SR(SS♀×RR♂)和反交F1RS(RR♀×SS♂)代的显性度DSR和DRS分别为0.40和0.60,表明抗性由不完全显性基因控制;两个D值(DRS和DSR)95%置信限有重叠,并且经t检验,两个D值不存在显著差异(P0.05),证明截形叶螨对哒螨灵的抗性遗传为常染色体控制;回交F2代(BC1RS和BC1SR)的实际死亡率和期望值经χ2检验,无显著性差异(χ2=9.72,df=9,P0.05),表明抗性遗传由单基因控制。

关 键 词:双生病毒鉴定  基因组变异  系统发育
收稿时间:2014/12/26 0:00:00
修稿时间:2015/3/24 0:00:00

Resistance inheritance patterns to pyridaben in Tetranychus truncatus
Song Liwen,Zhang Junxi,Yang Shunyi,Shen Huimin.Resistance inheritance patterns to pyridaben in Tetranychus truncatus[J].Plant Protection,2016,42(1):112-115.
Authors:Song Liwen  Zhang Junxi  Yang Shunyi  Shen Huimin
Institution:1. Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and ForestrySciences, Beijing 100097, China; 2. Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
Abstract:Tomato yellow leaf curl disease (TYLCD) is an important disease of tomato caused by many geminiviruses. A geminivirus was found in 25 of the 51 samples collected in Beijing, and complete genome sequences of 10 isolates were determined. All of the sequences contained 2 781 nucleotides encoding six proteins and shared more than 99% nucleotide identity with Tomato yellow leaf curl virus-Israel (TYLCV-IL). Genetic analysis based on the genome sequence of TYLCV isolates in China revealed that TYLCV had little genetic variability, and phylogenetic analyses showed that they clustered into three groups.
Keywords:identification of geminivirus  genetic variation of genome  phylogenetic analysis
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