| 鹤顶兰四倍体植株的诱导与鉴定 |
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| 引用本文： | 李豆豆,刘芝龙,黄明忠,陈积天,唐燕琼,杨光穗.鹤顶兰四倍体植株的诱导与鉴定[J].园艺学报,2013,40(10):2033-2038. |
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| 作者姓名： | 李豆豆 刘芝龙 黄明忠 陈积天 唐燕琼 杨光穗 |
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| 作者单位： | （1海南大学热带作物种质资源保护与开发利用教育部重点实验室，海南大学农学院，海口 570228；2中国热带农业科学院热带作物品种资源研究所，海南儋州 571737） |
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| 摘 要： | 以鹤顶兰原球茎为材料，使用秋水仙素进行了四倍体的离体诱导。结果表明，采用秋水仙素浸泡法可以诱导鹤顶兰原球茎发生倍性变化，含0.02%秋水仙素的“花宝一号”溶液浸泡处理6 d诱导率最高，达22.5%。细胞学鉴定发现，诱变成活植株中有3株为四倍体，其植株形态、气孔大小、气孔密度与二倍体存在差异，细胞核明显增大。
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| 关 键 词： | 鹤顶兰 秋水仙素 四倍体 原球茎 |
| 收稿时间： | 2013-07-05 |
Induction and Determination of Tetraploid Plants of Phaius tankervilleae |
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| LI Dou-dou,LIU Zhi-long,HUANG Ming-zhong,CHEN Ji-tian,TANG Yan-qiong,YANG Guang-sui,.Induction and Determination of Tetraploid Plants of Phaius tankervilleae[J].Acta Horticulturae Sinica,2013,40(10):2033-2038. |
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| Authors: | LI Dou-dou LIU Zhi-long HUANG Ming-zhong CHEN Ji-tian TANG Yan-qiong YANG Guang-sui |
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| Institution: | （1Key Laboratory of Protection and Development Utilization of Tropical Crop Gemplasm Resources，Hainan University，Ministry of Education；College of Agronomy，Hainan University，Haikou 570228，China；2Tropical Crops Genetic Resources Institute，Chinese Academy of Tropical Agricultural Sciences，Danzhou，Hainan 571737，China） |
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| Abstract: | Tetraploids of Phaius tankervilleae were induced through the treatment of protocorms with colchicine in vitro. The results showed that ploidy levels of protocorms changed with colchicine soaking and the highest induction rate of 22.5% was obtained by immersing protocorms into 0.02% colchicine solution for 6 days. Cytological determination of regenerated plants indicated that three tetraploid plantlets were obtained among mutated plants survived，and the morphology，the number and size of stomata of the mutants were different from those of the diploid plants，with obviously bigger nucleus. |
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| Keywords: | Phaius tankervilleae Phaius tankervilleae colchicine tetraploid protocorm |
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