新疆天山地区弯刺蔷薇居群遗传多样性AFLP分析

对新疆天山地区弯刺蔷薇(Rosa beggeriana)居群进行遗传多样性分析,采用8对AFLP分子标记在5个居群中共检测到191个位点,其中多态性位点47个;各居群平均多态位点百分率为22.72%,Nei's基因多样性指数和Shannon指数分别为0.0904和0.1319,表明弯刺蔷薇存在丰富的遗传多样性。各居群遗传分化系数为0.1333,基因流为3.2513,表明居群间具较高的基因交流,遗传分化水平低,遗传变异主要存在于居群内。遗传相似度和聚类分析显示,各居群间遗传一致性很高,遗传距离较近,亲缘关系较近,并基本上依据地理距离进行聚类。通过与其它野生蔷薇比较分析发现,环境差异对于弯刺蔷薇居群的影响并不明显,其遗传多样性水平相对于其他物种较低,居群间的基因交流频繁,且遗传变异相对较小。

AFLP-based Genetic Diversity of Rosa beggeriana Populations in Tianshan Mountains of Xinjiang

The genetic diversity of Rosa beggeriana in Eastern Tianshan mountains of Xinjiang were analyzed based on AFLP markers. There were 191 loci with 47 polymorphic loci among 5 populations of R. beggeriana amplified by 8 pairs of AFLP markers. The average percentage of polymorphic loci（PPL），Nei’s gene diversity（h）and Shannon information index（I）among the populations were 22.72%，0.0904 and 0.1319，respectively，reflecting a quite abundant genetic diversity among the populations. The gene differentitation（Gst）and estimate of gene flow（Nm）were 0.1333 and 3.2513，respectively，indicating the genetic variation mostly existed within the populations due to the frequent gene flow among the populations. The genetic similarity and cluster analysis further showed that five populations were of high genetic identity and close relationship，which were classified mainly according to the geographic distances.Moreover，the genetic diversity of R. beggeriana did not much influence by the environment，and exhibited a lower genetic diversity level when compared with other wild rose species.