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| 春兰炭疽病致病菌Colletotrichum boninense的鉴定及生物学特性分析 | |
| 引用本文： | 周平兰,唐新科,龙岳林,周小毛. 春兰炭疽病致病菌Colletotrichum boninense的鉴定及生物学特性分析[J]. 园艺学报, 2015, 42(10): 1993-2001. DOI: 10.16420/j.issn.0513-353x.2015-0084 |
| 作者姓名： | 周平兰 唐新科 龙岳林 周小毛 |
| 作者单位： | 1湖南农业大学园艺园林学院，长沙 410128；2湖南农业大学植物保护学院，长沙 410128；3湖南省农业科学院农业生物技术研究中心，长沙 410125；4湖南科技大学生命科学学院，湖南湘潭 411201 |
| 基金项目： | 湖南省科技厅重点项目（2010NK2007） |
| 摘 要： | 以湖南野生春兰(Cymbidium goeringii)引种驯化过程中发生的炭疽病病株为试验材料,分离、鉴定病原菌,分析营养因子和非营养因子对病原菌生长的影响。结果表明:春兰炭疽病病原菌为博宁炭疽菌(Colletotrichum boninense Moriwaki,SatoTsukiboshi);适合生长的培养基有PDA、PSA和CSA;能有效利用多种碳源,最适合的为葡萄糖,有利生长的有机氮为酵母膏和蛋白胨,无机氮为硝酸钾;光照与黑暗对菌丝生长的影响无显著性差异;适宜p H 5.0~8.0;在10~35℃均能生长,最适温度为25℃;处理20 min,菌丝和孢子的致死温度分别为48和50℃。 |
| 关 键 词： | 春兰 炭疽病 Colletotrichum boninense 生物学特性 |
Identification of Pathogen Colletotrichum boninense from Cymbidium goeringii and Its Biological Characteristics |
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| ZHOU Ping-lan,,TANG Xin-ke,LONG Yue-lin,,ZHOU Xiao-mao,,. Identification of Pathogen Colletotrichum boninense from Cymbidium goeringii and Its Biological Characteristics[J]. Acta Horticulturae Sinica, 2015, 42(10): 1993-2001. DOI: 10.16420/j.issn.0513-353x.2015-0084 | |
| Authors: | ZHOU Ping-lan TANG Xin-ke LONG Yue-lin ZHOU Xiao-mao |
| Affiliation: | 1College of Horticulture and Landscape，Hunan Agricultural University，Changsha 410128，China；2College of Plant Protection，Hunan Agricultural University，Changsha 410128，China；3Center of Biotechnology Research，Hunan Academy of Agricultural Sciences，Changsha 410125，China；4School of Life Science，Hunan University of Science and Technology，Xiangtan，Hunan 411201，China |
| Abstract: | Pathogen causing anthracnose on Cymbidium goeringii in Hunan was isolated and identified，and the impact of nutritional and non-nutritional factors on its growth was analyzed. The results showed that the pathogen of anthracnose on Cymbidium goeringii was Colletotrichum boninense Moriwaki，Sato & Tsukiboshi. This strain grew well on media of PDA，PSA and CSA. For carbon sources，it grew better in glucose than in sucrose，maltose or fructose. Yeast extract，peptone and KNO3 were the better organic and inorganic nitrogen sources，respectively. The optimal acidity of culture medium was pH 5.0–8.0，light or darkness. Its hyphae could extend at 15–35 ℃，and the optimum temperature was 25 ℃. The lethal temperatures of hyphae and spores were 48 ℃ and 50 ℃ in 20 min，respectively. |
| Keywords: | Cymbidium goeringii anthracnose Colletotrichum boninense biological characteristic |
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