苹果MdCBL家族基因表达分析及MdCBL1的功能鉴定

利用生物信息学的方法,对10个苹果MdCBLs家族蛋白的功能域进行预测,并与拟南芥AtCBLs家族的10个蛋白进行了系统进化分析,同时对苹果MdCBLs基因进行了系统的命名。利用qRT-PCR,检测了苹果MdCBLs基因在不同组织的表达及对非生物胁迫(包括盐、低温、ABA、干旱)的响应,结果表明,MdCBLs在苹果不同组织及非生物胁迫中起重要作用。另外,通过遗传转化苹果愈伤组织鉴定了MdCBL1在盐胁迫中的功能,MdCBL1过量表达明显提高了转基因愈伤组织的盐胁迫抗性。

Classification and Expression Analysis of Apple MdCBL Family Genes with a Functional Characterization of MdCBL1

In this study，the functional domains of apple MdCBL family proteins were predicted with bioinformatics methods. Their phylogenetic relationship with 10 Arabidopsis AtCBL proteins were analyzed. They were systematically named based on the similarity to their Arabidopsis counterparts，respectively. Subsequently，Real-time quantitative RT-PCRs were performed to determine the expression levels of apple MdCBLs in different tissues and in response to various abiotic stresses including salt，low temperature，ABA and drought. Results demonstrated that MdCBLs may play roles in different tissues and be involved in the response to abiotic stresses in plants. Finally，MdCBL1 was genetically transformed into apple callus. Salt-tolerance assay indicated that overexpression of MdCBL1 remarkably increased the tolerance of transgenic apple callus to high salinity，further supporting that MdCBLs genes participate in the response of plants to abiotic stress.