大白菜突变体库的构建及M2叶片表型变异的研究

 利用不同浓度甲基磺酸乙酯（EMS）诱变大白菜自交系‘A03’种子，根据M₁代植株发芽率、成活率、育性及M₂代成活率，筛选出适宜的EMS诱变浓度为0.4%。采取EMS种子诱变1次、EMS种子诱变2次及EMS花蕾诱变结合小孢子培养的方法构建了含有4 253个家系及相应自交M₂代种子的大白菜突变体库。在该突变体库M₂群体中分别对苗期6 404株幼苗及莲座期7 756个单株的叶片性状进行了形态学调查，总的表型变异频率高达37.62%和26.33%。

Construction of One Mutant Library and Research on Phenotypic Variation of M2 Population Leaves in Chinese Cabbage

Seeds of the Chinese cabbage inbred line‘A03’were treated by EMS with different concentrations in this study. The suitable EMS concentration for seed mutagenesis was determined as 0.4% by studying the germination rate，survival rate，fertility of M1 plants and survival rate of M2 individuals. By three ways of one time seeds treatment with EMS，two times of seeds treatment with EMS，and buds treatment with EMS combined with microspore culture，the Chinese cabbage mutant library contained 4 253 M1 families and their selfing seeds of M2 was constructed. For M2 individuals in the mutant library，leaf phenotypic traits of 6 404 seedlings at seedling stage and 7 756 plants at rosette stage were investigated，resulting in total mutant frequency of 37.62% and 26.33%，respectively.