利用荧光标记SSR构建百合种质资源分子身份证

 以96份百合种质资源为材料，使用SSR标记进行百合种质分子身份证构建试验。从172对百合SSR引物中筛选出20对多态性好的核心引物，采用四重荧光毛细管电泳技术检测扩增条带分子量的方法对百合资源进行标记分析，获得供试样品相应的扩增带型。采用个位数字和小写英文字母对不同带型进行编码，按照20对引物扩增带型数由少到多顺序串联排序的方式构建供试样品的分子身份证。结果显示：20对SSR引物共检测到69个SSR等位位点和170个带型，平均每对引物对品种扩增的等位位点3.45个，带型8.50个。对带型进行编码的结果表明：96份百合种质资源的SSR分子身份证互不相同，可以将材料完全区分开。

Using the Fluorescent Labeled SSR Markers to Establish Molecular Identity of Lily Germplasms

In this paper，96 lily germplasms collected from different countries were used to established molecular identity by simple sequence repeat（SSR）markers. In this research，20 of 172 SSR primer pairs，with high polymorphisms and good repeatability，were selected，and then were labeled with four kinds of fluorescent for amplification and capillary electrophoresis. The products of 96 samples with each primer pairs were analyzed. Each band pattern were coded by different single digit or lowercase letters. Meanwhile，according to the number of band patterns，from less to more，the order of 20 primer pairs were decided，and molecular identities of 96 lily germplasms were established. The results showedthat a total of 69 polymorphic alleles and 170 polymorphic patterns were revealed by the 20 primer pairs，with an average of 3.45 alleles and 8.50 patterns for each primer pairs，and all molecular identities of germplasms are different. This study indicated that the detection technology by using fluorescent labeled SSR markers had merits of reliable，efficient and high-throughput，and that it is convenient and efficient to establish the molecular identities of lily gemplasms using the above encoded mode of patterns.