| 1型鸭肝炎病毒基因组全长cDNA克隆的构建 |
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| 引用本文： | 于可响,沙明利,林树乾,姜亦飞,黄兵.1型鸭肝炎病毒基因组全长cDNA克隆的构建[J].水禽世界,2011(10):10-13. |
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| 作者姓名： | 于可响 沙明利 林树乾 姜亦飞 黄兵 |
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| 作者单位： | 山东省农业科学院家禽研究所 |
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| 基金项目： | 山东省自然科学基金项目资助(Y2008D55) |
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| 摘 要： | 参考1型鸭肝炎病毒基因组序列，设计7条引物，用RT—PCR方法扩增出覆盖整个病毒基因组三个忠实性片段，并按顺序组装进载体pBR322中，获得全长cDNA克隆。测序结果表明，该克隆与母本毒序列同源性达99．6％，并且5’端的T7启动子和3’端的Mlu I线性化位点均成功引入。
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| 关 键 词： | 1型鸭肝炎病毒 RT—PCR 全长cDNA克隆 |
Construction of the full-length cDNA Clone of Duck Hepatitis Virus Type 1 |
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| YU Ke-xiang,SHA Ming-li ,LIN Shu-qian,JIANG Yi-fei,HUANG Bing.Construction of the full-length cDNA Clone of Duck Hepatitis Virus Type 1[J].Poultry Science,2011(10):10-13. |
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| Authors: | YU Ke-xiang SHA Ming-li LIN Shu-qian JIANG Yi-fei HUANG Bing |
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| Institution: | (Institute of Poultry, Shandong Academy of Agricultural Sciences, Jinan 250023, China) |
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| Abstract: | Seven primers were synthetized according to genome sequence of duck hepatitis virus(DHV)type1, and three fidelity DNA fragments covering the full genome of DHV type1 were amplified by RT-PCR, and these fragments were inserted into pBR322 vector according to the genome sequence, then gained the full-length cDNA clone.Sequence homology is 99.6% with the clone and female parent virus, and T7 promotor and MluⅠsite are respectively added to genome 5’ end and 3’ end. |
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