不同西兰花品种中硫代葡萄糖苷的组分与含量分析

采用 HPLC 法测定分析了29个西兰花品种的硫代葡萄糖苷组分与含量，结果检测到8种硫代葡萄糖苷，包括4种脂肪族硫苷：3-甲基硫氧烯丙基硫苷、2-羟基-3-丁烯基硫苷、4-甲基硫氧丁基硫苷和3-丁烯基硫苷；4种吲哚族硫苷：4-羟基吲哚基-3-甲基硫苷、3-甲基吲哚基硫苷、4-甲氧基吲哚基-3-甲基硫苷和1-甲氧基吲哚基-3-甲基硫苷。总硫苷含量平均为11.27μmol/g，RAA、GBC 和 NEO 是西兰花三种主要的硫苷成分，分别占总硫苷含量的34.78%、25.02%和23.25%。不同西兰花品种的总硫苷含量变异范围5.60～17.04μmol/g，存在较大差异。

Glucosinolate Component and Content Analysis of Different Broccoli Varieties

Glucosinolates component and content of 29 broccoli varieties were analyzed by HPLC method. As a result, a total of eight kinds of glucosinolates in broccoli bud-head were detected. Four kinds of them were aliphatic glucosino-lates, such as glucoiberin(IBE), progoitrin(PRO), glucoraphanin(RAA) and gluconapin(NAP), and the others were indolic glucosinolates, such as 4-Hydroxyglucobrassicin(4OH), glucobrassicin(GBC), 4-Methoxyglucobrassicin(4ME) and Neoglu-cobrassicin (NEO). The content of total glucosinolates was 11.27μmol·g-1 DW. RAA, GBC and NEO were the predomi-nant component of glucosinolates in broccoli bud-head, with a proportion of 34.78%, 25.02% and 23.25% respectively. The content of total glucosinolates in different variety of broccoli varied from 5.60 μmol·g-1 DW to 17.04 μmol·g-1 DW.