BABA,BTH对番茄白粉病的抗性研究

分别以500 mg·L-1BABA和50 mg·L-1BTH两种诱导剂处理番茄幼苗，后期进行白粉病菌的接种，接种时间分别为诱导剂处理后1，2，3，5，8和15 d。通过病情指数、相对防效、H2O2和NO含量以及抗白粉病相关基因表达水平的测定，比较分析两种诱导剂的作用时间、最优处理模式以及抗病的相关机制。结果表明：BABA处理后防治效果最佳的间隔时间为第2天，且有效持续时间为10 d左右，BTH处理后1，3和5 d均为最佳期间且有效持续时间可达15 d，相对BABA持续期较长；BABA和BTH诱导后H2O2和NO含量均有显著增加；两种诱导剂处理后抗白粉病相关基因CHI3，GLUCA，GLUCB和PR1A表达趋势相近，但不同抗病相关基因在不同处理时间以及不同材料中均存在差异；喷施BABA和BTH后均有助于番茄抵抗白粉病菌的侵染，提高番茄的抗病能力。

Study on the resistance induced by BABA and BTH against powdery mildew in tomato

The tomato seedlings were treated with 500 mg·L-1 BABA and 50 mg·L-1 BTH respectively， then inoculated with powdery mildew fungus on 1， 2， 3， 5， 8 and 15 d after treatment. The duration of action， optimal processing mode and disease related mechanisms of two inducers were investigated by measuring disease index， relative control effect, H2O2 and NO content and powdery mildew resistance related gene expression levels. The results showed that the best time of BABA was the second day and the effective duration was about 10 days， the best time of BTH was the first， third and fifth day and the effective duration was up to 15 days. The contents of H2O2 and NO were significantly increased. The expression patterns of CHI3， GLUCA， GLUCB， PR1A genes all showed similar trend， but had differences at different treatment time in different materials. In conclusion， BABA and BTH could be used to improve the disease resistance of tomato to tomato powdery mildew.