| 苏云金杆菌δ-内毒素基因(Bt)导入苹果 |
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| 引用本文： | 程家胜,田颖川,孟秀美,李文谷,刘旭锋,张景亭,莽克强.苏云金杆菌δ-内毒素基因(Bt)导入苹果[J].西北农业学报,1999,8(1):78-81. |
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| 作者姓名： | 程家胜 田颖川 孟秀美 李文谷 刘旭锋 张景亭 莽克强 |
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| 作者单位： | [1]陕西省果品研究中心 [2]中科院微生物所植物生物技术开放实验室 |
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| 摘 要： | 应用携带改造过的长约１．８ｋｂ抗虫基因Ｂｔ的农杆菌ＬＢＡ４４０４对苹果品种绿袖、金矮生和长富２号的叶片外植体通过真空渗入接种、共培养和在含５０ｍｇ／Ｌ卡那霉素的培养基上筛选７０ｄ，获得对卡那霉素具抗性、存活并产生愈伤组织的外植体为１６．７％￣３７．３％，其中１８．７％￣９２．９％的外植体经噻重氮苯基脲（ＴＤＺ）诱导后，即产生不定芽和形成试管绿苗营养系，营养系经过多聚酶链反应（ＰＣＲ）测定表明，约有
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| 关 键 词： | 苹果 Bt基因 农杆菌 基因导入 苹小卷叶蛾 |
| 收稿时间： | 1998/1/5 0:00:00 |
| 修稿时间： | 1998/4/8 0:00:00 |
Transfer Bt-gene to Apple |
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| Cheng Jiasheng,Tian Yingchuan,Meng Xiumei,Li Wengu,Liu Xufeng,Zhang Jingting and Mang Keqiang.Transfer Bt-gene to Apple[J].Acta Agriculturae Boreali-occidentalis Sinica,1999,8(1):78-81. |
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| Authors: | Cheng Jiasheng Tian Yingchuan Meng Xiumei Li Wengu Liu Xufeng Zhang Jingting and Mang Keqiang |
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| Institution: | Shaanxi Fruit Crops Research Center, Xi''an 710065;The Research Institute of Microbiology CAS, Beijing 100080;Shaanxi Fruit Crops Research Center, Xi''an 710065;Shanghai Research Institute of Entomology CAS, Shanghai 200025;Shaanxi Fruit Crops Research Center, Xi''an 710065;Shaanxi Fruit Crops Research Center, Xi''an 710065;The Research Institute of Microbiology CAS, Beijing 100080 |
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| Abstract: | Leaf pieces from in vitro-grown shoots of 3 apple cultivars were contaminated by Agrnbacterium tumefaciens strain LBA4404 containing the binary vector pBin438 or pB 48.7 carrying modefied about 1.8 kb Bt-gene. The contamination has been done on condition of vaccume. After cocultivated with the agrobacterium for 3 days and selected by kanamycin for 70 days. The rate of explants forming callus is 16%~37%.The explants with callus have been induced by Thidiazuron (TDZ) to form adventitious buds and to grow out new shoots. The rate of induction is about 18%~92% on different cultivars. According to PCR test,over 50% of regenerated clones perform positively. It is confirmed that the Bt-gene has transformed into apples. However,only a few transformed apples have weak ability of resistance to tortrix moth(Ado.rophyes cnngrana Walke). |
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| Keywords: | Apple Agrobacterium Gene transformation Bt-gene Tortrix month |
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