A simplified spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners |
| |
Authors: | Los Weinert Patrícia Pezza Helena Redigolo de Oliveira José Eduardo Pezza Leonardo |
| |
Affiliation: | Instituto de Química, UNESP, P.O. Box 355, 14801-970, Araraquara, SP, Brazil. |
| |
Abstract: | ![]() A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (lambda(max) = 550 nm). Beer's law is obeyed in a concentration range of 2.05 x 10(-4) to 3.00 x 10(-3) M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 x 10(-5) M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2-104.3% with standard deviations ranging from to 0.5-1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|