鳗鲡肌肉中孔雀石绿代谢物隐性孔雀石绿染料残留标准物质的研制

为建立鳗鲡肌肉中孔雀石绿代谢物隐性孔雀石绿染料残留标准物质的研制和定值方法,以一定质量浓度孔雀石绿对鳗鲡进行药浴给药,使孔雀石绿在鱼体内自然代谢,从而使鳗鲡体内含有隐性孔雀石绿残留。经均质、真空包装及辐照处理后,获得一批500个独立包装的的鳗鲡肌肉样本。采用超高效液相色谱-串联质谱法对该样本进行均匀性和稳定性检验,经8家独立实验室协同定值及不确定度评估,其特性值为2.82μg/kg,扩展不确定度为0.39μg/kg(k=2)。所建立的制备方法为染料残留鳗鲡基体标准物质的实验室制备提供了一种参考。

Development of a Reference Material for Leucomalachite Green Residues in Eel Muscle

Eel (A nguilla anguilla) was bathed in malachite green at certain concentration to make the malachite green enter the eel and contain leucomalachite green (LMG) in muscle via metabolism to estab‐lish a method for the preparation and certification of the reference material of leucomalachite green (LMG) residues in the muscle .The positive 500 bags of samples of eel were performed in one batch by the proce‐dure of homogenation ,vacuum packing and irradiation .The homogeneity and stability of samples were de‐tected by ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS) .The con‐centration of the chemical constituent of the sample was certified through the collaborative analysis pro‐gram participated by 8 laboratories ,and the uncertainty assessment was performed with property value of 2 .82 μg/kg ,and the expanded uncertainty of 0 .39 μg/kg (k= 2) .It is concluded that the preparation method for matrix reference materials for the dye residues provides one kind of reference .