High level of resistance to Sclerotinia sclerotiorum in introgression lines derived from hybridization between wild crucifers and the crop Brassica species B. napus and B. juncea |
| |
Authors: | Harsh Garg Chhaya Atri Prabhjodh S Sandhu Balvir Kaur Michael Renton Shashi K Banga Hardeep Singh Charandeep Singh Martin J Barbetti Surinder S Banga |
| |
Institution: | 1. School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia;2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004 Punjab, India;3. CSIRO Sustainable Ecosystems, Floreat, WA 6014, Australia;4. Department of Agriculture and Food Western Australia, Baron-Hay Court, South Perth, WA 6151, Australia |
| |
Abstract: | Sclerotinia rot caused by the fungus Sclerotinia sclerotiorum is one of the most serious and damaging diseases of oilseed rape and there is keen worldwide interest to identify Brassica genotypes with resistance to this pathogen. Complete resistance against this pathogen has not been reported in the field, with only partial resistance being observed in some Brassica genotypes. Introgression lines were developed following hybridization of three wild crucifers (viz. Erucastrum cardaminoides, Diplotaxis tenuisiliqua and E. abyssinicum) with B. napus or B. juncea. Their resistance responses were characterized by using a stem inoculation test. Seed of 54 lines of B. napus and B. juncea obtained from Australia, India and China through an Australian Centre for International Agricultural Research (ACIAR) collaboration programme were used as susceptible check comparisons. Introgression lines derived from E. cardaminoides, D. tenuisiliqua and E. abyssinicum had much higher levels (P < 0.001) of resistance compared with the ACIAR germplasm. Median values of stem lesion length of introgression lines derived from the wild species were 1.2, 1.7 and 2.0 cm, respectively, as compared with the ACIAR germplasm where the median value for stem lesion length was 8.7 cm. This is the first report of high levels of resistance against S. sclerotiorum in introgression lines derived from E. cardaminoides, D. tenuisiliqua and E. abyssinicum. The novel sources of resistance identified in this study are a highly valuable resource that can be used in oilseed Brassica breeding programmes to enhance resistance in future B. napus and B. juncea cultivars against Sclerotinia stem rot. |
| |
Keywords: | Erucastrum cardaminoides Diplotaxis tenuisiliqua E abyssinicum Brassicas Introgression lines Resistance White mold |
本文献已被 ScienceDirect 等数据库收录! |
|