| 水稻基因启动子Ospz1的克隆与表达特性研究 |
| |
| 引用本文： | 赵洋,杨细平,王曼玲,李落叶,夏新界.水稻基因启动子Ospz1的克隆与表达特性研究[J].农业现代化研究,2011,32(2):234-237. |
| |
| 作者姓名： | 赵洋 杨细平 王曼玲 李落叶 夏新界 |
| |
| 作者单位： | 1. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南,长沙,410125;中国科学院研究生院北京,100049
2. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南,长沙,410125 |
| |
| 基金项目： | 中国科学院"百人计划"项目 |
| |
| 摘 要： | 在水稻基因组芯片分析的基础上,克隆到一个在水稻中高水平表达基因OsSG15'末端启动子区域1.6-kb的DNA片断,即Ospz1启动子,构建了由Ospz1启动子引导的GUS重组基因,并经农杆菌介导将重组基因导入到水稻中.对转基因水稻植株中GUS活性的定性测定结果表明,Ospz1启动子可驱动GUS报告基因在转基因水稻植株叶片、芽和根中高效表达,而在其它器官中不表达或表达活性极弱,表现出组织特异性.Ospz1启动子可用于农作物生物技术的遗传改良.
|
| 关 键 词： | 水稻 启动子Ospz1 转基因水稻 GUS活性 组织特异性 遗传改良 |
Cloning and Analysis of Rice Gene Promoter Ospz1 |
| |
| ZHAO Yang,YANG Xi-ping,WANG Man-ling,LI Luo-ye and XIA Xin-Jie.Cloning and Analysis of Rice Gene Promoter Ospz1[J].Research of Agricultural Modernization,2011,32(2):234-237. |
| |
| Authors: | ZHAO Yang YANG Xi-ping WANG Man-ling LI Luo-ye and XIA Xin-Jie |
| |
| Institution: | Key Laboratory of Agro-ecological processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Graduate University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Agro-ecological processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Graduate University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Agro-ecological processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Key Laboratory of Agro-ecological processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Key Laboratory of Agro-ecological processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China |
| |
| Abstract: | To clone a rice gene promoter, a highly active gene, OsSG1, was selected from rice based on the data obtained by microarray analysis. A 1.6-kb DNA fragment, Ospz1, at the 5' promoter region of the gene was cloned and sequenced. In order to investigate the promoter activity in transgenic rice, Ospz1 was ligated to the GUS gene in vector pCAMBIA1301, and the constructed vector was delivered into rice by Agrobacterium-mediated method. GUS histochemical staining assay showed that Ospz1 directed the GUS gene expression in leaves, sprouts and roots of transgenic rice plants, but not in other organs. Ospz1 is valuable and may be used as a promoter in the genetic improvement of crops through biotechnology. |
| |
| Keywords: | rice promoter Ospz1 transgenic rice plants GUS staining assay tissue-specific genetic improvement |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《农业现代化研究》浏览原始摘要信息 |
| 点击此处可从《农业现代化研究》下载免费的PDF全文 |
|
