| Pti5基因植物双元表达载体的构建及鉴定 |
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| 引用本文： | 张艳华,王罡,季静,杜娟,张领兵,王军军. Pti5基因植物双元表达载体的构建及鉴定[J]. 吉林农业大学学报, 2002, 24(1): 18-20 |
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| 作者姓名： | 张艳华 王罡 季静 杜娟 张领兵 王军军 |
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| 作者单位： | 解放军军需大学植物分子生物学研究室,吉林,长春,130062 |
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| 基金项目： | 国家植物转基因中试及产业化基地”专项基金资助项目 (J99 B 0 0 1) |
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| 摘 要： | Pto基因编码丝氨酸／苏氨酸蛋白激酶,对番茄细菌病具有良好抗性。Pti5是与Pto互作的蛋白质,Pti5蛋白与广泛存在的病程相关蛋白基因（PR基因）中的顺式元件相结合,可增强PR基因的表达,为提高植物抗病性提供了有效的途径。本研究将Pti5基因构建到植物双元表达载体pBI121上,获得pBI121UCH1重组质粒,并将该质粒转到根癌农杆菌LBA4404中,为植物利用根癌农杆菌转化系统转Pti5基因进行抗病基因工程育种奠定了基础。
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| 关 键 词： | Pti5基因 PR基因 抗病基因 表达载体 植物 鉴定 基因表达 构建 |
| 文章编号： | 1000-5684(2002)01-0018-03 |
| 修稿时间： | 2001-05-11 |
Construction and Identification of Plant Expression Vector Pti5 Gene |
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| ZHANG Yan hua,WANG Gang,JI Jing,DU Juan,ZHANG Ling bing,WANG Jun jun. Construction and Identification of Plant Expression Vector Pti5 Gene[J]. Journal of Jilin Agricultural University, 2002, 24(1): 18-20 |
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| Authors: | ZHANG Yan hua WANG Gang JI Jing DU Juan ZHANG Ling bing WANG Jun jun |
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| Abstract: | Pto gene encodes a serine /threonine protein kinase that confers resistance to tamato bacterial speck disease Pti5 gene encodes protein that interacts with Pto Pti5 protein specifically recognizes and combines with a cis element which is present in the promoter region of a large number of genes encoding "pathogenesis related"(PR)proteins In this way Pti5 can enhance the expression of PR genes and provide a new approach to the improvement of disease resistance of crops In this research, Pti5 gene was inserted into plant expression vector pBI121, and recombinant plasmid pBI121UCH1 was obtained The recombinant plasmid was transformed to Agrobacterium tumefaciens LB4404, which provides a new vector for the transformation of Pti5 gene by Agrobacterium transformation system |
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| Keywords: | PR gene resistance gene expression vector |
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