应用血液蛋白遗传标记研究广东鸡种类缘性

用聚丙烯酰胺凝胶电泳法和醋酸纤维素薄膜电泳法检测广东鸡地方品种杏花鸡、胡须鸡和清远麻鸡的血浆淀粉酶(Amy—1、Amy—2)、碱性磷酸酶(Akp—1、Akp—2)、酯酶(Es—1)、血红蛋白(Hb)等六种血液蛋白的多态型,计算各群体各位点的表型及基因频率,求出各品种间的相似系数、遗传距离和分比时间,用系统聚类法、模糊聚类法、Tocher聚类法和主坐标分析法研究广东地方鸡种的亲缘关系。研究结果表明:三个广东地方鸡种的血液蛋白表型及基因频率分布有很多相似之处,但又各有其特征;它们之间的亲缘关系都密切,其中杏花鸡与清远麻鸡的亲缘关系最为密切。

APPLYING BLOOD PROTEIN GENETIC MARKERS TO STUDYING THE CLASSIFICATIONS AND DIVERSIFICATIONS OF LOCAL CHICKEN BREEDS OF GUANGDONG

Genetic variants of six blood protein Barkers including plasma amylase(Amy-1 ang Amy-2),alkaline phosphatase(Akp-1 and Akp-2),esterase(Es-1)and hemo-globin(Hb)in Xinghua,Huiyang and Qingyuan chickens,the local breeds of Guangdong,China,were determined by Beans of polyacrytamide get electrophor-esis and cellulose acetate electrophoresls.Phenotypes distribution,gene frequencies,sinitarity coefficients,genetic distances and diversification time for these breeds were calculated.Using systemic cluster analysis,Fuzzy cluster analysis,Tocher cluster analysis and principal component analysis,the relationships among these Guangdong local chicken breeds were studied.The results showed much similarity among these three breeds,and some specific features in phenotype distribution and gene frequencies for every breed were found;and there were close relationships between them,the closest relationship being between Xinghua and Qingyuan chickens.