日粮限制法抑制甲基偶氮氧甲醇处理大鼠的肠道肿瘤发生

给雄性sprague-Dawley(S-D)大鼠接种一次甲基偶氮氧甲醇(Methylazoxymethanol,MAM),然后于140日内限制各组动物摄入日粮的数量,A组和B组在接种MAM后分别从第10天或第63天起每日仅给于日粮12克(25%限制)。C组和D组在接种MAM后分别从第8天或第31天起问日自由采食和禁食。对照大鼠用MAM处理后任其自由采食同一种日粮。当接种后第140天检查时,从第10天起限制日粮的A组,其肠道肿瘤显著减少;但其他各组间的肿瘤发病率并无显著差异。将每天都限制日粮的开始时间推迟,或间日自由采食或饥饿均不能改变肿瘤发生的模式,和自由采食对照组的肿瘤发生率并无差异。

Inhbition of Intestinal Tumorigenesis in Methylazoxymethanol-Treated rats by Dietary Restriction 1,2

Male Sprague-Bawley rats were inoculated once with methylazoxymethanol(MAM)and groups were fed on restricted diets for the following 140 days : Group A and B was fed on a 12—g diet daily (25％ restricted) from Day 10 and Day 63 after MAM, respeetively. Group C and D was fed ad libitum only every other day from day 8 or day 31 after MAM, respectively. Control MAM-treated rats were fed on the same diet ad Iibitum. When examined at day 140, there was a significant reducticn of intestinal tumors in Group A on the restricted diet from day 10; however, among all other groups there were no significant differences in tumor incidences. Later onset of daily dietary restriction or of ieeding and fasting overy other day did not change the Tattern of tumorigcnesis from that which developed in control rats fed ad libitum. (Cancer Treat Reports, 68( 2 ) : 405-408, 1984) 1. Received Feb. 17, 1983 ; revised July 15, 1983 ; accepted Sept. 1, 1983. 2. Reprint requests to:Dr. Morris Pollard, Lobund Laboratory, University of Xotre Dame, Notre Bame, IN 46556, USA,