| 传染性法氏囊病病毒JS株VP2基因真核表达载体的构建及其应用 |
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| 引用本文： | 刘红梅,秦爱建,许小琴,李迎晓,陈鸿军,叶建强,金文杰,刘岳龙,邵红霞. 传染性法氏囊病病毒JS株VP2基因真核表达载体的构建及其应用[J]. 中国预防兽医学报, 2006, 28(4): 461-465 |
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| 作者姓名： | 刘红梅 秦爱建 许小琴 李迎晓 陈鸿军 叶建强 金文杰 刘岳龙 邵红霞 |
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| 作者单位： | 1. 扬州大学,江苏省动物预防医学重点实验室,江苏,扬州,225009;安徽农业大学,动物科技学院,安徽,合肥,230036
2. 扬州大学,江苏省动物预防医学重点实验室,江苏,扬州,225009 |
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| 基金项目： | 高比容电子铝箔的研究开发与应用项目;全国高等学校优秀博士学位论文作者专项基金 |
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| 摘 要： | 应用RT-PCR方法从鸡传染性法氏囊病病毒(IBDV)JS株中扩增出VP2基因,并克隆入T-easy载体。序列测定分析结果表明IBDVJS株的VP2基因与国际标准强毒株的核苷酸序列同源性达98%,氨基酸序列同源性达99%以上。随后将VP2基因克隆入真核表达载体pcDNA3.1/zeo( ),构建成功真核表达质粒pcD-VP2,pcD-VP2体外转染COS-1细胞,能在COS-1细胞中表达。利用pcD-VP2质粒进行动物实验,结果表明雏鸡免疫14d后在体内可检测到特异性抗体,pcD-VP2的真核表达质粒二次免疫诱导鸡产生对IBDV强毒攻击的保护率为67%,这一结果提示VP2基因具有重要开发应用价值。
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| 关 键 词： | 传染性法氏囊病病毒 表达 |
| 文章编号： | 1008-0589(2003)04-0461-05 |
| 收稿时间： | 2005-04-06 |
| 修稿时间： | 2005-04-06 |
Construction of VP2 eukaryotic expression vector of infectious bursal disease virus and its application |
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| LIU Hong-mei,QIN Ai-jian,XU Xiao-qin,LI Ying-xiao,CHEN Hong-jun,YE Jian-qiang,JIN Wen-jie,LIU Yue-long,SHAO Hong-xia. Construction of VP2 eukaryotic expression vector of infectious bursal disease virus and its application[J]. Chinese Journal of Preventive Veterinary Medicine, 2006, 28(4): 461-465 |
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| Authors: | LIU Hong-mei QIN Ai-jian XU Xiao-qin LI Ying-xiao CHEN Hong-jun YE Jian-qiang JIN Wen-jie LIU Yue-long SHAO Hong-xia |
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| Affiliation: | 1. Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | The cDNA fragment of protective antigen VP2 gene of infectious bursal disease virus JS strain was amplified by RT-PCR,subcloned into pGEM-T easy vector and then into pcDNA3.1/zeo( )vector,designated as pcDVP2.By sequencing,the VP2 gene has high homology to that of very virulent IBDV strains.The transiently expression of VP2 in COS-1 cells was detected with the Mab specific to IBDV by the immunflurescent assay.After injection with pcDVP2 DNA to SPF chicken,the results showed that the specific anti-IBDV antibody could be detected in 14 days,67 % of the chickens were protected after challenge with IBDV JS strain. |
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| Keywords: | VP2 |
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