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| 甜樱桃体细胞胚胎发生及植株再生的研究 | |
| 引用本文： | 吴雅琴,赵艳华,刘国俭,李春敏. 甜樱桃体细胞胚胎发生及植株再生的研究[J]. 华北农学报, 2006, 21(Z2): 125-128 |
| 作者姓名： | 吴雅琴 赵艳华 刘国俭 李春敏 |
| 作者单位： | 河北省农林科学院昌黎果树研究所,河北,昌黎,066600 |
| 基金项目： | 河北省农林科学院科研基金 |
| 摘 要： | 以甜樱桃红灯为材料,幼果用0.1%HgCl2进行消毒,在无菌条件下取出未成熟胚子叶进行诱导,在胚成苗后进行继代和生根培养。培养结果表明,以MS为基本培养基,在2,4-D为2.0 mg/L时培养基上胚性愈伤组织诱导率较高,为53.7%;愈伤组织经过数次继代后转移至MS附加6-BA 1.0 mg/L和NAA 0.1 mg/L培养基上可诱导体细胞胚的形成,诱导率可达81.0%.体细胞胚在合适的培养基上可继续生长和增殖。 |
| 关 键 词： | 甜樱桃 愈伤组织 体细胞胚胎 植株再生 |
| 文章编号： | 1000-7091(2006)增刊-0125-04 |
| 修稿时间： | 2006-05-20 |
Somatic Embryogenesis of Sweet Cherry and Plantlet Regeneration |
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| WU Ya-qin,ZHAO Yan-hua,LIU Guo-jian,LI Chun-min. Somatic Embryogenesis of Sweet Cherry and Plantlet Regeneration[J]. Acta Agriculturae Boreali-Sinica, 2006, 21(Z2): 125-128 | |
| Authors: | WU Ya-qin ZHAO Yan-hua LIU Guo-jian LI Chun-min |
| Abstract: | Cultivars sweet cherry Hongdeng was used as the materials.An immature pterodia was taken from young fruit disinfected with 0.1% HgCl-2.Shoot multiplication culture and rooting culture were conducted after emergence of seedlings.The result indicates that the embryogenic callus(EC) frequency was 53.1.% at 2 mg/L of 2,4-D added in MS medium,After subcultured for several times,somatic embryogenesis at high frequency(81.0%) was obtained on MS medium supplemented with 6-BA 1.0 mg/L NAA 0.1 mg/L.Somatic embryos can keep on growing and proliferating on appropriate medium. |
| Keywords: | Sweet cherry Callus Somatic embryogenesis Plantlet regeneration |
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