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| 马流感病毒血凝素基因甲病毒复制子重组表达质粒的构建及其免疫效力评价 | |
| 引用本文： | 仇铮,郭巍,孙元,戚亭,仇华吉,相文华.马流感病毒血凝素基因甲病毒复制子重组表达质粒的构建及其免疫效力评价[J].中国预防兽医学报,2012,34(7):568-572. |
| 作者姓名： | 仇铮 郭巍 孙元 戚亭 仇华吉 相文华 |
| 作者单位： | 1. 东北农业大学动物医学院,黑龙江哈尔滨150030;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物传染病研究室,黑龙江哈尔滨150001 2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物传染病研究室,黑龙江哈尔滨,150001 3. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪病研究室,黑龙江哈尔滨,150001 |
| 基金项目： | 农业部公益性行业科研专项，2010年农业部马属动物疫病监测与诊断项目 |
| 摘 要： | 为评价马流感病毒(EIV)HA基因核酸免疫效果,本研究以甲病毒复制子载体pSFV1CS分别构建了表达EIV H3N8亚型的美洲型和欧洲型HA基因的重组真核表达质粒。并将其转染293T细胞,经间接免疫荧光鉴定表明HA基因获得表达;以重组质粒免疫的BALB/c鼠能够检测到特异性抗体产生,而且HI抗体水平持续升高,同时小鼠体内IFN-γ、IL-4分泌水平也有所升高。攻毒后小鼠表现轻度临床症状,但病毒分离和RT-PCR均未检测到病毒。上述结果表明,该重组质粒pSFV1CS-EIV-HA具有良好的免疫原性并且可以诱导免疫动物产生较高免疫应答的能力。 |
| 关 键 词： | 马流感病毒 甲病毒复制子载体 |
Immunization responses against equine influenza virus (EIV) based on recombinant Alphavirus replicon plasmid expressing EIV hemagglutinin gene |
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| QIU Zheng , GUO Wei , SUN Yuan , QI Ting , QIU Hua-ji , XIANG Wen-hua.Immunization responses against equine influenza virus (EIV) based on recombinant Alphavirus replicon plasmid expressing EIV hemagglutinin gene[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(7):568-572. | |
| Authors: | QIU Zheng GUO Wei SUN Yuan QI Ting QIU Hua-ji XIANG Wen-hua |
| Institution: | 1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;2.Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Harbin 150001,China;3.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultrual Sciences,Harbin 150001,China) |
| Abstract: | To evaluate DNA vaccination against equine influenza virus(EIV),the hemagglutinin(HA) genes of American and European H3N8 subtype EIV were amplified by RT-PCR and cloned into a Semliki forest virus(SFV) replicon based plasmid of pSFV to construct the recombinant plasmid pSFV1CS-EIV-HA,respectively.The HA expressions were identified in the 293T cells transfected with the recombinant plasmid by indirect immunofluorescence assay.Furthermore,the specific HI antibody,IFN-γ or IL-4 level were detected in BALB/c mice immunized with either single or both of the recombinant plasmids.However,the virus was failed to detect in immunized BALB/c mice by the RT-PCR detection or the virus isolation.The results indicated that the recombinant plasmids possessed a capability for inducing effective immune responses in animals,which was a promising candidate DNA vaccine against EIV infection. |
| Keywords: | equine influenza virus alphavirus replicon vector |
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