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| 内毒素对肠黏膜微血管内皮细胞内分泌一氧化氮和内皮素的影响 | |
| 引用本文： | 王 新,谭建华,刘金玲,韦旭斌,穆 祥.内毒素对肠黏膜微血管内皮细胞内分泌一氧化氮和内皮素的影响[J].中国农学通报,2006,22(1):4-4. |
| 作者姓名： | 王 新 谭建华 刘金玲 韦旭斌 穆 祥 |
| 作者单位： | 1. 吉林大学畜牧兽医学院临床系,吉林,长春,130062 2. 中国人民解放军军事医学科学院军事兽医研究所,吉林,长春,130062 3. 莱阳农学院动科系,山东,莱阳,265200 4. 北京农学院动科系,北京,102206 |
| 基金项目： | 北京市自然科学基金会重点资助项目，北京市教委资助项目“抗肠道大肠杆菌病中药方剂机理研究”(6021001)。 |
| 摘 要： | 目的是研究内毒素（lipopolysaccharide; LPS）导致肠黏膜微血管内皮细胞损伤的作用机制。将所培养的肠黏膜微血管内皮细胞分为空白对照组和LPS组，每组按时间分为4个亚组：3h、6h、9h、12h。空白对照组加维持培养液，LPS组加1μg/ml LPS培养液，在CO2培养箱内静置培养。结果表明，一氧化氮（NO）分泌明显升高，3h后达到高峰，随后逐渐降低，而内皮素（endothelin; ET）分泌急剧升高，9h后达到高峰，随后又开始逐渐降低，但一直保持在较高的水平。所以引起了NO和ET的升高可能是LPS导致肠黏膜微血管内皮细胞的损伤机制。 |
| 关 键 词： | 肠黏膜 微血管内皮细胞 LPS 损伤机制 影响 |
| 收稿时间： | 2005-09-25 |
| 修稿时间： | 2005-10-10 |
The Respective Effects of LPS on the Intestinal Mucosa Microvascular Endothelial Cells Secreting NO and ET |
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| Wang Xin,Tan Jianhu,Liu Jinling,Wei Xubin,Mu Xiang.The Respective Effects of LPS on the Intestinal Mucosa Microvascular Endothelial Cells Secreting NO and ET[J].Chinese Agricultural Science Bulletin,2006,22(1):4-4. | |
| Authors: | Wang Xin Tan Jianhu Liu Jinling Wei Xubin Mu Xiang |
| Institution: | 1.Clinic Department, Pasturage and Theriatrics School, Jilin University, Changchun Jilin 130062; 2.Institute of Military Veterinary Academy of Military Medical Science, PLA, Changchun Jilin 130062; 3.Foreign Language Department, Laiyang Agricuhural University, Laiyang Shandong 265200; 4.Animal Science Department, Beijing A gricuhural College, Beijing 102206 |
| Abstract: | The aim: To research on the functional mechanism of LPS damaging intestinal mucosa microvascular endothelial cells. The way: Divide the intestinal mucosa microvascular endothelial cells fostered into blank control group and LPS group and further divide each group into four minor groups according to the time: 3h, 6h, 9h, 12h. Add feeding medium into the blank control group while fill 1μg/mlLPS nutrient liquid into LPS group. After that, put them into CO2 incubators and cultivate them statically. Result: The secretion of NO rises obviously and arrives at the peak in three hours before it lowers gradually. The secretion of ET rises rapidly and arrives at the peak in 9 hours, then it begins to lower gradually, however, it always stays at a high level. Conclusion: the functional mechanism of LPS damaging intestinal mucosa microvascular endothelial cells may be that LPS leads to the rise in the secretion of NO and ET. |
| Keywords: | Intestinal mucosa Microvascular endothelial cells LPS Damaging mechanism Effect |
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