| 银杏细胞对抗生素的耐受性研究 |
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| 引用本文： | 刘小烛,铃木羲胜,山口勇. 银杏细胞对抗生素的耐受性研究[J]. 西南林学院学报, 2004, 24(2): 1-2,6 |
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| 作者姓名： | 刘小烛 铃木羲胜 山口勇 |
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| 作者单位： | 1. 西南林学院,资源学院,云南,昆明,650224
2. 理化学研究所,微生物制御研究室,日本,埼玉,和光,351-0198 |
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| 摘 要： | 用MS培养基诱导银杏胚产生愈伤组织．分别在MS培养基中加入不同浓度的卡那霉素、潮霉素和杀稻瘟菌素，将银杏愈伤组织转接到含抗菌素的培养基上继续培养11d之后得到以下结果：MS培养基中含潮霉素25mg／L时，银杏愈伤组织变成褐色，停止生长；MS培养基中含同样浓度的杀稻瘟菌素时，银杏愈伤组织变成浅褐色生长变慢；MS培养基中合同样浓度的卡那霉素时，银杏愈伤组织生长良好．银杏细胞对潮霉素的耐受性很弱，可以作为筛选转基因银杏树种的抗生素使用。
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| 关 键 词： | 银杏细胞 抗生素 耐受性 愈伤组织 培养基 |
| 文章编号： | 1003-7179(2004)02-0001-02 |
Tolerance of Ginkgo biloba Callus Cells with Antibiotics |
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| LIU Xiao-zhu,Yoshi Cuzuki,Isamu Yamaguchi. Tolerance of Ginkgo biloba Callus Cells with Antibiotics[J]. Journal of Southwest Forestry College, 2004, 24(2): 1-2,6 |
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| Authors: | LIU Xiao-zhu Yoshi Cuzuki Isamu Yamaguchi |
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| Affiliation: | LIU Xiao-zhu~1,Yoshi Cuzuki~2,Isamu Yamaguchi~2 |
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| Abstract: | The results obtained by having transplanted the callus of Ginkgo biloba produced from embryo on normal MS medium into mediums with different antibiotics for 11 days were as follows: (1)the callus cells turned brown and stopped growth on MS medium with 25 mg/L of hygromycin; (2) the callus cells turned light brown and partially stopped growth on MS medium with 25 mg/L blasticedin; (3) the callus cells of Ginkgo biloba grew well on MS medium containing 25 mg/L kanamycin. It showed that the callus cells of Ginkgo biloba were susceptible to hygromycin, and hygromycin could be used as antibiotics for screening of gene transferred varieties of Ginkgo biloba. |
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| Keywords: | Ginkgo biloba antibiotics tolerance |
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