Sequence Analysis of the Nucleoprotein Gene of Asian Lineage Peste des Petits Ruminants Vaccine Virus |
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| Authors: | D Muthuchelvan A Sanyal V Balamurugan P Dhar S K Bandyopadhyay |
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| Institution: | (1) Central Institute of Fisheries Technology, Cochin;(2) Project Directorate on Foot-and-Mouth Disease, IVRI Campus Mukteswar-Kumaon, Nainital;(3) Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital;(4) Animal Husbandry Commissioner, Krishi Bhavan, New Delhi, India |
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| Abstract: | The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96)
belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino
acids. The PPRV Sungri/96 N gene has a nucleotide homology of 92% for PPRV Nigeria 75/1 to 55.5% for canine distemper virus.
At amino acid level the homology was 94.1% with PPRV Nigeria 75/1, while with other morbilliviruses, PPRV Sungri/96 had only
71.4–64.9% amino acid identity. The phosphorylation prediction reveals eight conserved sites across morbilliviruses, whereas
in the C-terminal portion of the protein the sites are not conserved. Phylogenetic analysis of different N proteins of morbilliviruses
revealed five well-defined clusters as observed previously. To the best of our knowledge this is the first report describing
the nucleocapsid gene sequence of PPRV Indian isolate.
Muthuchelvan, D., Sanyal, A., Balamurugan,V., Dhar, P. and Bandyopadhyay, S.K., 2006. Sequence analysis of the nucleoprotein
gene of Asian lineage peste des petits ruminants vaccine virus.Veterinary Research Communications, 30(8), 957–963 |
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| Keywords: | Morbillivirus peste-des-petits ruminants virus vaccine virus N gene nucleotide sequence amino acid sequence |
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