乌拉尔甘草胚状体的诱导及再生体系的建立

用L9(34)正交试验设计方法研究了乌拉尔甘草外植体、植物生长调节剂对胚状体诱导的影响,并对胚状体诱导和成苗技术进行了探索。结果表明:适宜乌拉尔甘草胚状体诱导培养的最佳外植体为芽,优化培养基为MS+甘氨酸0.3g/L+蔗糖30g/L+琼脂5g/L+2,4-D0.2mg/L;2,4-D对胚状体诱导具有促进作用。适宜胚状体诱导成苗的最佳外植体为芽,培养基为MS,成苗率达到66.67%,外源激素对胚状体诱导成苗具有抑制作用。

SOMATIC EMBRYOID GENESIS INDUCTION AND PLANT REGENERATION in vitro IN Glycyrrhiza uralensis Fisch

Using the orthogonal experiment design of L9(34), the effects of explants uralensis, hormone types and combinations on inducing embryoids, embryoids induced seedling cultivation and cultivation techniques were researched. The results showed that the best explant was the shoot of Glycyrrhiza uralensis cultured by embryoid induction and the optimal induction medium was MS+glycine 0.3g/L+sucrose 30g/L+agar 5g/L+2,4-D 0.2mg/L. The obvious promotion of 2,4-D to embryoid induction was observed. The shoot was the best explant which could induce the seedling from the embryoid and the plantlet regeneration rate was 66.67% with the medium of MS. The exogenous hormone inhibited the induction of plantlet from embryoid.