苏云金芽胞杆菌工程菌BIOT185对金龟子幼虫的田间防效及其安全性评价

为评价苏云金芽胞杆菌(Bacillus thuringiensis,Bt)工程菌BIOT185对金龟子幼虫的田间防治效果以及其中间试验水平的安全性,在河北省定州市花生田开展了两年的田间小区试验。调查结果表明,每mL含100亿芽胞的工程菌BIOT185悬浮剂稀释10倍和100倍处理对金龟子幼虫具有较好的防治效果,虫口减退率可达52.08%～70.51%,保果效果为50.40%～66.57%,说明高浓度的BIOT185可以有效控制金龟子幼虫的危害。利用平板菌落计数法结合PCR检测发现,在施药后的365 d中BIOT185在土壤中的菌量总体呈下降趋势;调查田间各小区的昆虫种群发现,工程菌BIOT185处理区与野生菌HBF-1、BT185以及对照区的昆虫种群差异不显著。说明BIOT185不会在土壤环境中大量残留,也不会影响花生田的昆虫种群。

Control efficacy of genetically engineered strain BIOT185 against larvae of Scarab and its safety evaluation in peanut field

Field plot trials was conducted to evaluate control efficacy of Bacillus thuringiensis engineered BIOT185 against larvae of Scarab and its ecological safety in peanut field in Dingzhou,Hebei Province.Results showed that the numbers of Scarab decreased by 52.08%-70.51% and the nut-retention rate reached to 50.40%-66.57% after treated by BIOT185 suspension concentration (SC) at 10-fold and 100-fold dilution which showed that high concentrations of BIOT185 SC (100 hundred million/mL) could effectively control larvae of Scarab.The residual numbers of BIOT185 in soil showed downward trend in one year after application,detecting by methods of dull colony notation and PCR.Field investigation showed that the insect population in BIOT185 treated plot was no significant difference with that in control and wild strain HBF-1 and BT185 treated plot.These results demonstrated that engineered strain BIOT185 would not remain in the soil environment,and would not affect insect population in peanut fields.