| 蝴蝶兰ACC氧化酶和ACC合成酶融合反义表达载体的构建 |
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| 引用本文： | 王宇腾,崔波,蒋素华,武思,牛苏燕,叶永忠.蝴蝶兰ACC氧化酶和ACC合成酶融合反义表达载体的构建[J].河南农业科学,2011,40(6):115-117,121. |
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| 作者姓名： | 王宇腾 崔波 蒋素华 武思 牛苏燕 叶永忠 |
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| 作者单位： | 1. 河南农业大学生命科学学院,河南郑州,450002
2. 郑州师范学院生物工程研究所,河南郑州,450044 |
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| 摘 要： | 为了获得具有超常花期的蝴蝶兰新品系,根据已报道的蝴蝶兰ACC合成酶(ACS)基因和ACC氧化酶(ACO)基因的序列,设计引物从蝴蝶兰总RNA中克隆出ACS保守区的461 bp片段和ACO的333 bp片段,完成2个基因的克隆和测序后,将ACS基因片段和ACO基因片段以反义的方向插入到中间载体pBI221 CaMV启动子...
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| 关 键 词： | 蝴蝶兰 ACC氧化酶 ACC合成酶 融合反义基因 根癌农杆菌 |
Construction of Double-antisense Expression Vector of ACC Oxidase and ACC Synthase Gene in Phalaenopsis |
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| WANG Yu-teng,CUI Bo,JIANG Su-hua,WU Si,NIU Su-yan,YE Yong-zhong.Construction of Double-antisense Expression Vector of ACC Oxidase and ACC Synthase Gene in Phalaenopsis[J].Journal of Henan Agricultural Sciences,2011,40(6):115-117,121. |
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| Authors: | WANG Yu-teng CUI Bo JIANG Su-hua WU Si NIU Su-yan YE Yong-zhong |
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| Institution: | 1(1.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China;2.Institute of Bioengineering,Zhengzhou Normal University,Zhengzhou 450044,China) |
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| Abstract: | According to the reported ACC oxidase(1-aminocyclopropane-1-carboxylate oxidase,ACO) gene and ACC synthase(ACS) gene cDNA sequences,two pairs of specific primers were designed and synthesized,which were obtained from total RNA of Phalaenopsis by RT-PCR.The cDNA fragments were linked into a plant binary vector pBI221 between the CaMV 35S promoter and nos terminator.Then the recombinant fragment including ACC,ACO and 35S promoter was cloned into plant expression vector pCAMBIA3301 in orientation.The positive clones were detected by PCR technique and analyzed by the restriction enzyme.The constructs were transferred into agrobacterium LBA4404 via frozen-fusion method and had been used to transform Phalaenopsis protocorm by agrobacteria. |
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| Keywords: | Phalaenopsis hybrid ACC oxidase gene ACC synthase gene Double-antisense RNA Agrobacterium tumefaciens |
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