分子蒸馏耦合脂肪酶催化浓缩菜籽油脱臭馏出物中维生素E研究

【目的】菜籽油脱臭馏出物是天然维生素E的重要来源之一。为了从菜籽油脱臭馏出物中获得较高纯度的天然维生素E。【方法】采用分子蒸馏和脂肪酶催化浓缩菜籽油脱臭馏出物中维生素E；在分子蒸馏预处理和单因素试验的基础上，采用响应曲面法建立脂肪酶催化反应生成的脂肪酸甲酯含量的数学模型。【结果】当20g反应原料，反应温度37℃，初始加入水量11.77 ml，加入酶量30.78 U，水解时间16 h，在后续反应中单次加入甲醇量1.32 ml和脂肪酶15 U, 共加3次，总反应时间40 h，多次分子蒸馏后维生素E的纯度达到25%。【结论】分子蒸馏耦合脂肪酶催化浓缩菜籽油脱臭馏出物中维生素E取得了较好的效果,为其工业应用提供了基础。

Molecular Distillation and Enzymatic Reaction for Concentration of Vitamin E from Rapeseed Oil Deodorizer Distillate

Rapeseed oil deodorizer distillate was an important source of natural vitamin E. Molecular distillation and enzymatic reaction were applied for concentration of vitamin E. The model of content of fatty acid methyl ester produced by enzymatic reaction was presented by response surface methodology (RSM) on the basis of molecular distillation and single factor experiments . The results indicated that when material 20g, reaction temperature 37℃,the amount of water 11.7ml and Candida rugosa lipase 30.78 U for hydrolysis time 16h followed by amount of methanol added 1.32ml with lipase 15 U per time of three times, and total reaction time 40h, the content of vitamin E was 25% after twice molecular distillation.