| 应用重组PCR构建猪源膜联蛋白A2和增强型绿色荧光蛋白融合基因 |
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| 引用本文： | 陈江涛,吉艳红,王光祥,尚佑军,刘艳红,刘湘涛. 应用重组PCR构建猪源膜联蛋白A2和增强型绿色荧光蛋白融合基因[J]. 西北农业学报, 2010, 19(1): 38-43 |
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| 作者姓名： | 陈江涛 吉艳红 王光祥 尚佑军 刘艳红 刘湘涛 |
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| 作者单位： | 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室农业部畜禽病毒学重点开放实验室,兰州,730046 |
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| 基金项目： | 国家科技支撑计划重大项目 |
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| 摘 要： | 膜联蛋白A2是膜联蛋白多基因家族成员之一,在Ca2+存在时能与带负电荷的磷脂结合。最近研究显示,膜联蛋白A2在病毒感染方面发挥了重要作用。本研究应用RT-PCR和PCR方法分别获得了猪源膜联蛋白A2和增强型绿色荧光蛋白基因(EGFP)。然后用重组PCR技术成功构建了膜联蛋白A2-EGFP融合基因,为研究膜联蛋白A2在病毒感染细胞中的定位和表达,以及病毒和宿主细胞相互作用奠定了基础。
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| 关 键 词： | 重组PCR 膜联蛋白A2 增强型绿色荧光蛋白 融合基因 |
| 收稿时间： | 2009-04-25 |
| 修稿时间： | 2009-09-24 |
Construction of Fusion Gene of Enhanced Green Fluorescent Protein with Annexin A2 from Pig by Recombinant PCR |
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| CHEN Jiangtao,JI Yanhong,WANG Guangxiang,SHANG Youjun,LIU Yanhong and LIU Xiangtao. Construction of Fusion Gene of Enhanced Green Fluorescent Protein with Annexin A2 from Pig by Recombinant PCR[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(1): 38-43 |
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| Authors: | CHEN Jiangtao JI Yanhong WANG Guangxiang SHANG Youjun LIU Yanhong LIU Xiangtao |
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| Affiliation: | CHEN Jiangtao,JI Yanhong,WANG Guangxiang,SHANG Youjun LIU Yanhong , LIU Xiangtao(Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,National Foot-,-Mouth Diease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China) |
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| Abstract: | Annexin A2 is a member of the multigene family of annexin proteins,which are characterised by binding to negatively charged phospholipids in the presence of Ca2+.Recent studies show that annexin A2 playes a critical role in virus infection.Annexin A2 from pig and enhanced green fluorescent protein were amplified and gained by RT-PCR and PCR respectively.Then the fusion gene of enhanced green fluorescent protein with annexin A2 from pig was constructed by recombinant PCR.This work was necessary for the study... |
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| Keywords: | Recombinant PCR Annexin A2 Enhanced green fluorescent protein Fusion gene |
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