| Abstract: | Prohibitin is an antiproliferative protein that is a product of a putative tumor
suppressor gene. However, there is little information on prohibitins in companion animals.
In this study, we cloned canine prohibitin mRNA using RT-PCR and 3′-RACE (Rapid
Amplification of cDNA Ends). The sequence was well conserved compared with those of other
mammals, including human. The deduced amino acid sequence translated from the open reading
frame completely corresponded to the human sequence. Canine prohibitin mRNA was expressed
in all normal mammary and tumor samples examined. These results suggest that this protein
plays a vital role in cell growth mechanisms and may be related to the occurrence of
canine mammary tumors. |
