| 送春与多花兰杂种的非共生萌发与快速繁殖 |
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| 引用本文： | 周丽,胡春根.送春与多花兰杂种的非共生萌发与快速繁殖[J].北方园艺,2009(3). |
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| 作者姓名： | 周丽 胡春根 |
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| 作者单位： | 1. 华中农业大学,园林学院,湖北,武汉,430070;黔西南民族师专化生系,贵州,兴义,562400
2. 华中农业大学,园林学院,湖北,武汉,430070 |
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| 摘 要： | 用地生兰送春和附生兰多花兰进行种间杂交,对杂交种子进行非共生萌发和快速繁殖研究.结果表明:胚龄7个月、0.1 mol/L KOH处理10 min的种子萌发率最高;种子萌发后原球茎的增殖以Z4 (KC+BA 0.4 mg/L+NAA 0.6 mg/L+KT 0.2 mg/L ) 培养基为最好,增殖系数可达到12.8;在原球茎增殖和分化成苗过程中加入活性碳能够有效地防止切口褐化.
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| 关 键 词： | 杂种 非共生萌发 胚 原球茎 |
Asymbiotic Germination and Rapid Propagation of Hybrid of Cymbidium cyperifolim var.szechuanicum and C.floribundum |
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| ZHOU Li,HU Chun-gen.Asymbiotic Germination and Rapid Propagation of Hybrid of Cymbidium cyperifolim var.szechuanicum and C.floribundum[J].Northern Horticulture,2009(3). |
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| Authors: | ZHOU Li HU Chun-gen |
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| Institution: | 1.Huazhong Agricultural University;College of Horticulture and Forestry Science;Wuhan;Hubei 430070;China;2.Southwest Guizhou Teachers College for Nationalities;Xingyi;Guizhou 562400;China |
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| Abstract: | Interspecific hybridization was carried out between terrestrial orchid Cymbidium cyperifolim var.szechuanicum and epiphytic orchid C.floribundum.to study on the asymbiotic germination and rapid propagation of hybrid seeds.The results showed that Seven-month-embryo-aged-seeds had the highest germination rate,when treated with 0.1 mol/L KOH for 10 min.After germination,the best medium for PLBs propagation was Z4(KC+BA 0.4 mg/L + NAA 0.6 mg/L+KT 0.2 mg/L),the rate of PLBs proliferation could reach 12.8.Adding ... |
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| Keywords: | Hybrid Asymbiotic germination Embryo Protocorm-like body |
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