高产几丁质酶的粘质沙雷氏菌株的诱变选育

以黑龙江省科学院微生物研究所实验室保存的1株粘质沙雷氏菌S68为出发菌株,进行原生质体紫外线、Na NO2和复合诱变,通过透明圈对比和酶活测定,最终获得1株产酶量高于原始菌株4.3倍的粘质沙雷氏菌诱变菌株,产酶量达到4.98μg·h-1。确定该菌株最佳诱变方法为复合诱变,首先进行紫外诱变(高度为30 cm,诱变时间为12 s),再用化学诱变(0.4 mol·L-1Na NO2诱变5 min),经验证该诱变菌株遗传稳定。

HighC hitinase of Se rratia marcescens Strain Mutation

With Serrta ia marcescens S68 in the Heilongjiang Academy of Sciences Institute of Microbiology Laboratory , we ob-tained an enzyme production 4.3 times higher than the original strain of S.marcescens mutant strain by using protoplast ul-traviolet, NaNO2 and composite mutagenesis, with the enzyme production of 4.98μg·h -1.We determined that the best methods of mutagenesis of the strain was the composite mutagenesis by first UV mutation ( height of 30 cm, for mutagenesis time of 12 s), and then the chemical mutagenesis (0.4 mol· L-1 NaNO2 mutagenesis for 5 min).