Expression and significance of fgl2 in a model of rat coronary microthrombosis in situ

AIM: To explore the expression and significance of fibrinogen-like protein 2 prothrombinase/fibroleukin (fgl2) in a model of rat coronary microthrombosis. METHODS: Seventy-eight Sprague-Dawley male rats were randomly assigned to model group (n=36), sham operation group (n=36) for different time (1 h, 1 d, 1week, 2 weeks, 3 weeks, 4 weeks)(n=6, respectively), and normal control group (n=6). After closing aorta, sodium laurate was injected into aortic root of the animals in model group. The animals in sham operation group were injected with normal saline for control. Microthromb were detected with HE staining and modified martius-sarlet-blue stains (MMSB). von Willebrand factor (vWF) was measured with ELISA. fgl2 mRNA and protein expressions were detected by RT-PCR and immunohistochemistry staining, respectively. RESULTS: Microthromb were observed in rat coronary microvasculature. vWF was increased in plasma. fgl2 was induced in model group compared to sham operation group (P<0.05), no significant changes were observed in sham operation group and normal control group (P>0.05). CONCLUSION: The functional disorder of endothelial cells induces the expression of fgl2 in the coronary microvasculature. fgl2 prompts the thrombosis with platelet and fibrin.