Protective effects of nmhaFGF on NRK52E cell apoptosis induced by H2O2 |
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Authors: | HAI Guang-fan XU Hua YU Jing SU Zhi-jian ZHENG Qing XU Hong |
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Institution: | 1.Pharmacy Faculty of Xinxiang Medical College, Xinxiang 453003, China;2.Pharmacy College of Jinan University, Guangzhou 510632, China. E-mail: haiguangfan@163. com |
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Abstract: | AIM: The present study was designed to establish H2O2-induced NRK52E cell apoptotic model and to investigate the effects and the mechanism of nmhaFGF on the NRK52E cell apoptosis induced by H2O2. METHODS: In vitro experiment, a apoptotic model of normal rat kidney epithelium (NRK52E) was made by MTT method, Hoechst33342 dyeing and flow cytosorting (FCR). NRK52E cells were cultured with different concentrations of nmhaFGF and haFGF for 24 h before H2O2 was added. The apoptotic rate was detected with FCR method. RESULTS: H2O2 at concentration of 0.4 mmol/L, the optimal condition to establish the apoptotic model, was used to treat NRK52E cells for 18h. Different doses of nmhaFGF (0.01, 0.03, 0.10, 0.30, 1.00 mg/L) reduced the apoptotic rates with the dose rising. However, the decreasing tends of apoptotic rates with dose increasing for haFGF was not so obvious. CONCLUSION: nmhaFGF protects the NRK52E cells against apoptosis. The mechanism might be connected with its non-mitogenic property. |
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