Suprahepatic vena cava manipulative bleeding alleviates liver ischemia-reperfusion injury

AIM: To determine the time course and peak production of reactive oxygen species (ROS) in suprahepatic vena cava (SH-VC) after hepatic ischemia-reperfusion(HIR) and to study the effects of posthepatic manipulative bleeding on HIR injury in rat model. METHODS: Rat 35 min total hepatic ischemia model was used in this study. Blood was taken, from SH-VC and infrahepatic vena cava (IH-VC), for MDA detection at 0 min, 5 min, 10 min, 30 min, 1 h, 2 h and 6 h after reperfusion respectively. Then the experimental rats were treated with posthepatic manipulative bleeding or IH-VC manipulative bleeding at 10 min after reperfusion. RESULTS: MDA concentration in SH-VC elevated obviously with time and peaked at 10 min after reperfusion. Two percent of body weight posthepatic manipulative bleeding with blood transfusion at 10 min after reperfusion significantly decreased circulating MDA，ALT and AST levels and improved survival rate. CONCLUSION: ROS concentration in SH-VC elevates obviously after reperfusion. Two percent of body weight suprahepatic vena cava manipulative bleeding with blood transfusion at 10 min after reperfusion can afford significant protection against warm HIR injury.