Comparison of Clostridioides difficile strains from animals and humans: First results after introduction of C. difficile molecular typing and characterization at the Istituto Zooprofilattico Sperimentale of Piemonte,Liguria e Valle d’Aosta,Italy |
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| Institution: | 1. S.C. Sicurezza e Qualità degli Alimenti, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;2. Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy;3. S.C. Diagnostica Generale, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;4. Laboratorio di Microbiologia e Virologia, Azienda Ospedaliero-Universitaria, Città della Salute e della Scienza di Torino, Turin, Italy;5. Dipartimento di Ispezione degli Alimenti, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (NA), Italy;6. S.C. Epidemiologia ed Analisi del Rischio, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;1. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Microbiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania;2. Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary;3. Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07, Uppsala, Sweden;4. SLU Global Bioinformatics Centre, Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07, Uppsala, Sweden;5. Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, 1425, Ciudad Autónoma de Buenos Aires, Argentina;6. Instituto de Agrobiotecnología y Biología Molecular, IABiMo, INTA-CONICET, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, Castelar, 1712, Buenos Aires, Argentina;1. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, 1419963111, Iran;2. Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, 1419963111, Iran;3. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, 1419963111, Iran;4. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. UGC Microbiología, Hospital Universitario Puerta del Mar, Avda. Ana de Viya 21, 11009, Cádiz, Spain;2. Instituto de Investigación e Innovación Biomédica de Cádiz (INIBICA), Hospital Universitario Puerta del Mar, Avda. Ana de Viya 21, 11009, Cádiz, Spain;3. Universidad de Cádiz, Cádiz, Spain |
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| Abstract: | PCR ribotypes (RTs027 and 078) are known causes of Clostridioides difficile infection (CDI) in humans. Molecular typing and characterization of 39 C. difficile strains isolated from samples from humas and animals in 2016–2018 indicated an overlap of RTs between community-acquired patients (CA-CDI) and domestic animals from the same geographical area; 14 RTs were identified: 12 RTs were positive for toxins A/B; RT078, RT080 and RT126 were also positive for binary toxin (CDT). Most of the RTs from the animals (RTs020, 078, 106, 126) were also detected in the samples from humans. Strains grouped into three clusters: cluster I included prevalently human strains, mainly RT 018; clusters II and III included strains from humans and animals, mainly RT078 and RT020. The CA-CDI strains suggested animals as a reservoir of C. difficile isolated together with other microorganisms from animals, highlighting the association of enteric pathogens as a cause of infection and death. |
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| Keywords: | Molecular characterization PCR-ribotyping One Health |
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