Serologic and molecular survey of horses to Coxiella burnetii in East of Iran a highly endemic area |
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| Institution: | 1. Department of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;2. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Microbiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, Serbia;2. Public Communal Company, Veterina Belgrade, Emergency Room Utility, Serbia;3. Department of Veterinary Medicine, College of Food and Agriculture, United Arab Emirates University (UAEU), Al Ain, P.O. Box 1555, United Arab Emirates;4. Department of Animal Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia;5. Department of Animal Husbandry, Faculty of Agriculture Kru?evac, University of Ni?, Serbia;6. Veterinary Station in ?abac j.s.c., Serbia;1. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Anhui Science and Technology University, Fengyang, Anhui, China;2. College of Animal Science, Anhui Science and Technology University, Fengyang, China;3. Laboratory of Pork Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Fengyang, Anhui, China;1. Department of Pathobiology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran;2. Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran;1. Department of Veterinary Medicine, University of Perugia, Via S. Costanzo 4, 06126 Perugia, Italy;2. Istituto Zooprofilattico Sperimentale of Umbria and Marche, Via Salvemini 1, 06126 Perugia, Italy;3. Institute of Epidemiology, Friedrich-Loeffler-Institute, Seestraße 55, 16868 Wusterhausen, Germany;1. Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;2. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;3. Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran |
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| Abstract: | There are few reports about Q fever in horse populations worldwide. This study aimed to detect the C. burnetii infection by serologic and molecular confirmation using commercial ELISA kit and real-time PCR in the East of Iran a region highly endemic. A total of 177 blood samples and 115 vaginal swabs were randomly collected from horses in East of Iran. The sera samples were analyzed for anti C.burnetii Ig G antibodies by a commercial ELISA kit and nucleic acid extraxted from vaginal samples were used to determine the C. burnetii DNA by real-time PCR assay. Antibodies were detected in 5.64 % (10/177) of sera samples and C. burnetii DNA was detected in 7.82 % (9/115) of horse vaginal samples. There was no significant difference in seroprevalence in different sex, age and breed groups. Our study showed that horses could be considered as a mild potential reservoir of C. burnetii which may be effective on horse health status. However, additional studies are needed to assess whether the horse could be considered as a relevant transmission risk indicator for Q fever. |
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| Keywords: | Serology Real-time PCR Horse Iran |
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