Evaluation of genetic variation in Lachenalia bulbifera (Hyacinthaceae) using RAPDs |
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Authors: | Riana Kleynhans Johan J Spies |
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Institution: | (1) ARC-Roodeplaat, Private Bag X293, Pretoria, 0001, South Africa;(2) Department of Botany and Genetics, U.O.F.S., P.O. Box 339, Bloemfontein, 9301, South Africa |
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Abstract: | RAPD (randomly amplified polymorphic DNA) analyses were carried out on 21 accessions of Lachenalia bulbifera (Cyrillo) Engl. Five pre-selected primers produced an average of 88% polymorphisms. Fifteen of the 21 accessions could be
identified using the five primers. In a pairwise comparison genetic distance values ranging from 0.11 to 1.08 were obtained.
These values reveal a high amount of variation within the species. The genetic distance values within the tetraploid and hexaploid
groups on the south coast were low, but values were high between the groups on the south coast and those on the west coast.
A dendogram was constructed from the RAPD banding profiles, using UPGM cluster analysis. The dendogram clusters certain accessions
together. These clusters are supported by their geographical locality and chromosome data. The hexaploid group, tetraploid
group and octoploid group on the south coast are respectively clustered together. It is concluded that RAPDs can be used to
assess the genetic variation at an intra-specific level in Lachenalia.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | genetic variation Lachenalia RAPD |
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