朱顶红‘Red Lion’胚性愈伤组织诱导及体细胞胚发生

以‘Red Lion’朱顶红(Hippeastrum vittatum)的鳞片为外植体进行体细胞胚诱导和形态学与组织学的胚性鉴定。结果表明,鳞片在MS+2 mg·L~(-1) BA+1 mg·L~(-1) NAA+2 mg·L~(-1) TDZ+30 g·L~(-1)蔗糖培养基上胚性愈伤组织诱导率可达90.63%,而不添加TDZ的培养基上胚性愈伤组织诱导率为0。胚性愈伤组织可呈白色松散、透明膨松、透明致密、微棕色松脆和翠绿色松脆等类型;非胚性愈伤组织呈微棕色致密状。胚性愈伤组织中可明显观察到球形胚、心形胚和棒状胚3个发育时期。体细胞胚在不添加植物生长调节剂的MS培养基上可发育形成芽和根,长成完整小植株。

The Embryonic Callus Induction and Somatic Embryogenesis of Hippeastrum vittatum‘Red Lion’

Somatic embryo induction and morphology as well as histology identification were carried on Hippeastrum vittatum‘Red Lion’by the way of scales explant. The results showed that the optimum medium for embryonic callus induction was MS + 2 mg · L^-1 BA + 1 mg · L^-1 NAA + 2 mg · L^-1 TDZ + 30 g · L^-1 sucrose，and the rate of embryonic callus induction reached to 90.63%. However，there was no embryonic callus obtained in medium without TDZ. On PGR-free medium，somatic embryos developed after 15 days and plantlets with shoot and roots formed after 30 days. There were 6 types of callus：white loose callus，transparent fluffy callus，transparent compact callus，light brown crisp callus and jade green crisp callus，for embryonic callus and light brown compact callus，for non-embryonic callus. Global embryos，heart-shaped embryos and club-shaped embryos were observed in the development process of somatic embryo.