| C、D型貂源铜绿假单胞菌的生物学特性鉴定及免疫效果评价 |
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| 引用本文： | 吕娇,闫明灏,韩坤,程悦宁,廉士珍,徐淑娟,史秋梅,陈丽凤,曲学忠,丛培强,赵辉,闫喜军,白雪.C、D型貂源铜绿假单胞菌的生物学特性鉴定及免疫效果评价[J].动物医学进展,2019(6):23-29. |
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| 作者姓名： | 吕娇 闫明灏 韩坤 程悦宁 廉士珍 徐淑娟 史秋梅 陈丽凤 曲学忠 丛培强 赵辉 闫喜军 白雪 |
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| 作者单位： | 吉林农业大学动物科学技术学院;中国农业科学院兰州兽医研究所;中国农业科学院特产研究所;河北科技师范学院动物科技学院;山东省威海市文登区畜牧兽医技术服务中心;敦化市畜牧局 |
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| 基金项目： | 国家自然科学基金青年科学基金(31502102);吉林省科技发展计划项目(20190401006NY) |
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| 摘 要： | 从辽宁和黑龙江2个水貂养殖场死亡水貂肺中分离出13株铜绿假单胞菌,血清型鉴定分别为C型和D型,命名为PK13-C和H2F-D。为了解其作为疫苗株的免疫效果,测定了其运动能力、绿脓菌素生成能力、生长曲线、毒力、免疫原性及耐药性。结果表明,PK13-C对小鼠和水貂的毒力分别为7.5×106CFU和7×105CFU,泳动能力及群集运动能力较强,抽动能力较弱,生物被膜形成能力中等;H2F-D对小鼠和水貂的毒力分别为2.05×107CFU和2.2×106CFU,泳动能力较弱,但群集运动及抽动能力较强,生物被膜形成能力较弱。二者对本血清型菌株的免疫保护率均为100%,对异种血清型菌株的保护率为80%~90%。2个株菌均可产生绿脓菌素。生长曲线显示2个菌株于4h进入对数生长期,于18h左右进入平稳期。药敏试验结果表明,2个株菌对氯霉素、复方新诺明、氨苄西林/舒巴坦耐药。
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| 关 键 词： | 铜绿假单胞菌 C型 D型 免疫原性 |
Identification of Biological Characteristics and Evaluation of Immune Effect of Pseudomonas Aeruginosa C and D Strains from Mink |
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| LV Jiao,YAN Ming-hao,HAN Kun,CHENG Yue-ning,LIAN Shi-zhen,XU Shu-juan,SHI Qiu-mei,CHEN Li-feng,QU Xue-zhong,CONG Pei-qiang,ZHAO Hui,YAN Xi-jun,BAI Xue.Identification of Biological Characteristics and Evaluation of Immune Effect of Pseudomonas Aeruginosa C and D Strains from Mink[J].Progress In Veterinary Medicine,2019(6):23-29. |
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| Authors: | LV Jiao YAN Ming-hao HAN Kun CHENG Yue-ning LIAN Shi-zhen XU Shu-juan SHI Qiu-mei CHEN Li-feng QU Xue-zhong CONG Pei-qiang ZHAO Hui YAN Xi-jun BAI Xue |
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| Institution: | (Jilin Agricultural University,Changchun,Jilin,130118,China;Lanzhou Veterinary Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730000,China;Institute of Special Animal and Plant Science of CAAS,Changchun,Jilin,130122,China;Institute of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei,066004,China;Animal Husbandry and Veterinary Technology Service Center,Wendeng,Shandong,264400,China;Dunhua Animal Husbandry Bureau,Yanbian Korean Autonomous Prefecture,Yanbian,Jilin,133700,China) |
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| Abstract: | LV Jiao;YAN Ming-hao;HAN Kun;CHENG Yue-ning;LIAN Shi-zhen;XU Shu-juan;SHI Qiu-mei;CHEN Li-feng;QU Xue-zhong;CONG Pei-qiang;ZHAO Hui;YAN Xi-jun;BAI Xue(Jilin Agricultural University,Changchun,Jilin,130118,China;Lanzhou Veterinary Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730000,China;Institute of Special Animal and Plant Science of CAAS,Changchun,Jilin,130122,China;Institute of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei,066004,China;Animal Husbandry and Veterinary Technology Service Center,Wendeng,Shandong,264400,China;Dunhua Animal Husbandry Bureau,Yanbian Korean Autonomous Prefecture,Yanbian,Jilin,133700,China) |
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| Keywords: | Pseudomonas aeruginosa type C type D immunogenicity |
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