利用基因芯片技术分析水稻杂种优势的分子机理

采用基因芯片技术,以超级杂交稻先锋组合两优培九及其亲本和分离世代F2代的优势集团和负优势集团为材料,尝试利用水稻表达谱芯片分析水稻杂种优势的分子机理.结果表明,以杂种F1(两优培九)为对照组,亲本9311、培矮64S以及F2代的优势集团、负优势集团的有效基因数分别为8 304,8 962,9 956,10 196个,差异表达基因数分别有447,122,617,388个,这些差异表达基因涉及代谢、发育、运输和蛋白修饰等多种功能,但其中大部分基因的功能尚未知.提出了差异表达基因的显性表达水平的概念.分析表明,具显性表达水平的差异表达基因的聚合与杂种优势表现无明显相关性.

Studies on Molecular Basis of Rice Heterosis by cDNA Microarray Analysis

Using Liangyou Peijiu (the pioneer super hybrid rice combination in China) and its parents and segregated F_2 population as the materials, the molecular basis of rice heterosis was analyzed through cDNA microarray expression of rice by way of microarray technology. The results showed that, using Liangyou Peijiu (F_1) as the check group, 9311 (male parent), Peiai 64S (female parent), predominance bulk (F_2) and negative predominance bulk (F_2) had 8 304, (8 962), 9 956 and 10 196 valid genes, among which the differential expression genes were 447, 122, 617 and 388, respectively. The functions of some differential expression genes were related to metabolism, growth, transportation, protein modification, etc., but the functions of most other differential expression genes were unknown. A new term of dominant expression level of differential expression genes was put forward. It was found that there was not an obvious correlation of the cumulation of differential expression genes with dominant expression level to the expression of rice heterosis.