粘帚霉对核盘菌菌核的寄生作用及其细胞壁降解酶活性分析

在培养皿中测定粘帚霉对核盘菌菌核的寄生作用,结果表明:不同粘帚霉菌株对核盘菌菌核具有较强的寄生作用,寄生率均达到85%以上。HL-1-1菌株的寄生率最高,为100%,其次为菌株SH-1-1、SYP-1-3和SS-1-1。供试粘帚霉菌株使核盘菌菌核软化,最后导致腐烂。平板透明圈法和比色法测定粘帚霉的细胞壁降解酶活性结果表明:供试的粘帚霉菌株菌能产生蛋白酶、几丁质酶、葡聚糖酶和纤维素酶,其寄生菌核能力越强,蛋白酶、几丁质酶和葡聚糖酶的酶活性越高。表明在粘帚霉侵染核盘菌菌核的过程中,蛋白酶、几丁质酶和葡聚糖酶起着重要作用。

Studies on the Cell Wall Degrading Enzymes during the Mycoparasitism of Gliocladium spp. Isolates with the Sclerotia of Sclerotinia sclerotiorum

Mycoparasitism of some isolates of Gliocladium spp.with the sclerotia of Sclerotinia sclerotiorum were studied.Results indicated that the isolates showed strong parasite ability with the sclerotia and the average of parasite rate were above 85%.Among them,the parasite rate of HL-1-1 strain was 100% and those of SH-1-1,SYP-1-3 and SS-1-1 followed.Mycoparasitism with the Gliocladium spp.isolates tested led the sclerotia issue soft and rot at the end.Some cell wall degrading enzymes including the proteinase,chitinase,glucanase and cellulase were induced.The higher the parasite ability is,the stronger the activity of the former three enzymes are.It showed that the proteinase,chitinase and glucanase played an important role during the mycoparasitism process.