| pH值对绿色木霉合成纤维素酶的影响 |
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| 引用本文： | 季更生,林弦,曹阳,朱婵.pH值对绿色木霉合成纤维素酶的影响[J].安徽农业科学,2007,35(27):8593-8594. |
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| 作者姓名： | 季更生 林弦 曹阳 朱婵 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018 |
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| 摘 要： | 以绿色木霉(Trichoderma viride)为产酶菌,研究了不同pH值控制方法对绿色木霉合成纤维素酶的影响。结果表明,初始pH值对绿色木霉合成纤维素酶的影响较大,当初始pH值为4.5时,纤维素酶活力到第7天达到40.74 IU/ml;而初始pH值为6.0时,纤维素酶活力到第7天仅有29.02 IU/ml。采用产酶过程调控pH值,当pH值7.5为最佳调控点,此时纤维素酶活力最高可达41.35 IU/ml。采用0.1mol/L的柠檬酸缓冲液调节产酶过程中的培养基pH值;经过6 d培养;纤维素酶活力可达58.70 IU/ml。
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| 关 键 词： | pH值 绿色木霉 纤维素酶 |
| 文章编号： | 0517-6611（2007）27-08593-02 |
| 修稿时间： | 2007-05-18 |
Effecs of pH Value on the Production of Cellucase from Trichoderma vivide |
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| JI Geng-sheng et al.Effecs of pH Value on the Production of Cellucase from Trichoderma vivide[J].Journal of Anhui Agricultural Sciences,2007,35(27):8593-8594. |
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| Authors: | JI Geng-sheng |
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| Institution: | Jiangsu University of Science and Technology ,Zhenjiang,Jiangsu 212018 |
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| Abstract: | The effects of pH value on production of cellulase from Trichoderma viride were investigated.The results showed that the cellulase activity was 40.74 IU/ml when the initial pH value of culture media was 4.5.And the cellulase activity was 29.02 IU/ml when the initial pH value of culture media was 6.0.While pH value of culture media was maintained at 7.5,the cellulase activity was 41.35 IU/mL.The maximal cellulase activity reached 58.70 IU/mL at the sixth day when the buffer solution of 0.1 mol/L citric acid was used to control pH value of culture media. |
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| Keywords: | pH value Trichoderma viride Cellulase |
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